Biomaterial Database

Stable isotopic tracers for studies of glucose metabolism. 1996

S C Kalhan

Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

Because of its unique metabolism, glucose illustrates the advantages and limitations of stable isotopic tracers for in vivo studies. The present review describes the application of stable tracers in studies of glucose metabolism. The natural abundance of these tracers requires that careful attention be given to the dose of the tracer and the impact of the background abundance on measurements. The consequence of the natural variation of 13C abundance on measurements in vivo is described, as is the application of multilabeled molecules to estimate kinetics and intrahepatic precursor pool enrichments. The final section outlines recently described methods of estimation of gluconeogenesis.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D002247	Carbon Isotopes	Stable carbon atoms that have the same atomic number as the element carbon but differ in atomic weight. C-13 is a stable carbon isotope.	Carbon Isotope,Isotope, Carbon,Isotopes, Carbon
D005943	Gluconeogenesis	Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man