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Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/microns carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing.
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
January 2020,
Methods in molecular biology (Clifton, N.J.),
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
January 2014,
Methods in molecular biology (Clifton, N.J.),
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
January 2005,
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Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
April 2015,
The Plant journal : for cell and molecular biology,
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
March 1994,
Carcinogenesis,
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
June 2011,
Nucleosides, nucleotides & nucleic acids,
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
April 1994,
Mutation research,
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
December 2000,
International journal of radiation biology,
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
January 2013,
Nucleosides, nucleotides & nucleic acids,
Y Kagawa, and
F Yatagai, and
M Suzuki, and
Y Kase, and
A Kobayashi, and
M Hirano, and
T Kato, and
M Watanabe, and
F Hanaoka
October 2017,
Mutation research,
