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Most nonmalignant cells are anchorage-dependent; they require substrate attachment for growth and, in some instances, survival. This requirement is lost on oncogenic transformation. The cyclin E-CDK2 complex, which is required for the G1-S transition of the cell cycle, was activated in late G1 phase in attached human fibroblasts, but not in fibroblasts maintained in suspension. In transformed fibroblasts the complex was active regardless of attachment. The lack of cyclin E-CDK2 activity in suspended cells appeared to result from increased expression of CDK2 inhibitors and a concomitant decrease in phosphorylation of CDK2 on threonine-160. Suppression of cyclin E-CDK2 activity may thus underlie the anchorage dependence of cell growth.
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
June 2000,
Development, growth & differentiation,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
August 2003,
Cell,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
November 1997,
Biochemical and biophysical research communications,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
February 1997,
Biochemistry and molecular biology international,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
March 2004,
Cell cycle (Georgetown, Tex.),
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
May 1998,
Oncogene,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
November 2011,
The Journal of biological chemistry,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
November 2000,
The EMBO journal,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
December 2000,
Oncogene,
F Fang, and
G Orend, and
N Watanabe, and
T Hunter, and
E Ruoslahti
November 1999,
Molecular and cellular biology,
