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The feasibility of using the Reuber H-35 rat hepatoma cell (RH-35 cells) as model for studying metallothionein induction was examined. The RH-35 cells were treated with Cd, a toxic metal which is known to induce metallothionein. The LC50 after a 3-h treatment was 70 microM. The value was significantly higher (P < 0.05) if the cells were pre-treated with a sublethal dose of CdCl2 (5 microM) for 2 days, indicating that pre-treatment with a low dose of Cd can protect against a subsequent higher dose of the same metal. Both the mRNA and the gene product metallothionein can be identified in the cells 2 days after treatment with 5 microM Cd. In addition to Cd, Zn and Cu were also able to induce the expression of metallothionein to various degrees. The results indicate that the MT gene is present in RH-35 cells and is responsive to treatment with various metals. Thus, this cell line can be used as a model to study metallothionein induction.
M S Yang, and
M K Tang, and
R N Wong
May 1971,
Cancer research,
M S Yang, and
M K Tang, and
R N Wong
May 1983,
Biochimica et biophysica acta,
M S Yang, and
M K Tang, and
R N Wong
February 1996,
Biology of reproduction,
M S Yang, and
M K Tang, and
R N Wong
January 1978,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
M S Yang, and
M K Tang, and
R N Wong
December 1982,
In vitro,
M S Yang, and
M K Tang, and
R N Wong
November 1979,
Molecular pharmacology,
M S Yang, and
M K Tang, and
R N Wong
April 1997,
Biology of reproduction,
M S Yang, and
M K Tang, and
R N Wong
January 1979,
Biochimie,
M S Yang, and
M K Tang, and
R N Wong
January 1976,
Journal of cyclic nucleotide research,
M S Yang, and
M K Tang, and
R N Wong
April 1987,
Biochemistry international,
