Biomaterial Database

Production and use of monoclonal antibodies to Microsporum canis. 1995

L Pinter, and H J Ellis, and P J Ciclitira, and W C Noble

Department of Microbiology and Infectious Diseases, Veterinary Faculty University of Zagreb, Croatia.

Microsporum canis NCPF 179 and M. canis NCPF 177 dermatophyte cytoplasmic extracts (DCEs) were used as antigens to generate monoclonal antibodies (mAbs). Hybridomas with supernatants of optical density (OD) > 1 for homologous dermatophyte cytoplasmic extracts (CE) and OD < 0.5 for heterologous CE of Candida albicans tested by the enzyme-linked immunosorbent assays (ELISA) were selected for cloning. mAbs secreted by cloned hybridoma lines were screened against CE of M. canis, M. gypseum, M. equinum, M. distortum, Trichophyton verrucosum, C. albicans, Malassezia pachydermatis and Aspergillus fumigatus. The ELISA performed on clone supernatants identified a variety of different activities between the dermatophyte and other fungal CEs. The selected mAbs were used for immunoblotting of native and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Immunoblots with some of the mAbs tested allowed the differentiation of strains belonging to different dermatophyte species and isolates belonging to the M. canis.

UI	MeSH Term	Description	Entries
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008864	Microsporum	A mitosporic Oxygenales fungal genus causing various diseases of the skin and hair. The species Microsporum canis produces TINEA CAPITIS and tinea corporis, which usually are acquired from domestic cats and dogs. Teleomorphs includes Arthroderma (Nannizzia). (Alexopoulos et al., Introductory Mycology, 4th edition, p305)	Microsporon,Microsporons,Microsporums
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006825	Hybridomas	Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.	Hybridoma
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000946	Antigens, Fungal	Substances of fungal origin that have antigenic activity.	Fungal Antigen,Fungal Antigens,Antigen, Fungal
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus