Biomaterial Database

Fractionation of purified nucleosomes on the basis of aggregation properties. 1977

M M Sanders, and J T Hsu

Monomeric nucleosomes from micrococcal nuclease digest of rat liver nuclei have been purified on Sepharose columns in the presence of divalent cations and 0.6 M NaCl. The particles contain histones H2A, H2B, H3, H4, and a complement of nonhistone chromosomal proteins. In 0.6 M NaCl, the nucleosome mixture sedimented at 10 S; however, when the NaCl was removed approximately 30% of the particles aggregated and precipitated, and the remaining soluble fraction sedimented at 11 S. The aggregation phenomenon was divalent cation-dependent and reversible. Characterization of the macromolecular components of the subfractions of nucleosomes showed that the subfractions differed in composition of species of histone H3 as well as of several nonhistone chromosomal proteins but not in the size of the DNA fragment present. The aggregation properties of the isolated nucleosomes showed similarities to the divalent cation-dependent differences in the extent of chromatin condensation in the intact eukaryote nucleus.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014783	Viscosity	The resistance that a gaseous or liquid system offers to flow when it is subjected to shear stress. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Viscosities
D046911	Macromolecular Substances	Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.	Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus