BIOMAT Database Logo BIOMAT Database Logo

Molecular characterization of the t(2;5) (p23; q35) translocation in anaplastic large cell lymphoma (Ki-1) and Hodgkin's disease. 1996

H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Department of Pathology, New York University Medical Center, New York, USA.

The precise cellular origin and the pathogenetic mechanism(s) leading to the neoplastic transformation of anaplastic large cell lymphoma (ALCL) and the Reed-Sternberg cell of Hodgkin's disease (HD) remains largely uncertain. Classical cytogenetic analysis has shown a unique translocation involving bands 2p23 and 5q35 bands in a variable number of ALCLs. It has been recently shown that the nucleophosmin/B23 (NPM) gene (5q35) and a novel anaplastic lymphoma kinase (ALK; 2p23) are the fused genes of t(2;5). To investigate the presence and the precise frequency of NPM-ALK gene products among ALCL and HD cases, a large and well-characterized panel of ALCL (n = 49) and HD (n = 72) cases was studied using multiple strategies including reverse transcriptase-polymerase chain reaction (RT-PCR), Southern blot analysis, and immunohistochemistry. Overall, 6 (3 T and 3 null) of 49 ALCL and 3 (2 nodular sclerosis and 1 mixed cellularity) of 72 HD showed the presence of NPM-ALK transcripts by RT-PCR. NPM-ALK gene rearrangements were detected in all RT-PCR, NPM-ALK-positive ALCL by Southern blot analysis. Furthermore, in all the available cases we were able to show the presence of ALK-related protein using a specific polyclonal antiserum recognizing the cytoplasmic domain of ALK by immunohistochemistry. Our data show that NPM-ALK gene transcripts are identified in a subpopulation of ALCL, almost exclusively in T or null cell in origin, and in rare cases of HD. These findings show that some HD may be closely related to ALCL, giving us new insights on the pathogenesis and possibly biologic evolution of HD.

UI MeSH Term Description Entries
D008215 Lymphocytes, Null A class of lymphocytes characterized by the lack of surface markers specific for either T or B lymphocytes. Null Cells,Cell, Null,Cells, Null,Lymphocyte, Null,Null Cell,Null Lymphocyte,Null Lymphocytes
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D010750 Phosphoproteins Phosphoprotein
D011505 Protein-Tyrosine Kinases Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors. Tyrosine Protein Kinase,Tyrosine-Specific Protein Kinase,Protein-Tyrosine Kinase,Tyrosine Kinase,Tyrosine Protein Kinases,Tyrosine-Specific Protein Kinases,Tyrosylprotein Kinase,Kinase, Protein-Tyrosine,Kinase, Tyrosine,Kinase, Tyrosine Protein,Kinase, Tyrosine-Specific Protein,Kinase, Tyrosylprotein,Kinases, Protein-Tyrosine,Kinases, Tyrosine Protein,Kinases, Tyrosine-Specific Protein,Protein Kinase, Tyrosine-Specific,Protein Kinases, Tyrosine,Protein Kinases, Tyrosine-Specific,Protein Tyrosine Kinase,Protein Tyrosine Kinases,Tyrosine Specific Protein Kinase,Tyrosine Specific Protein Kinases
D002889 Chromosomes, Human, Pair 2 A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification. Chromosome 2
D002895 Chromosomes, Human, Pair 5 One of the two pairs of human chromosomes in the group B class (CHROMOSOMES, HUMAN, 4-5). Chromosome 5
D004273 DNA, Neoplasm DNA present in neoplastic tissue. Neoplasm DNA
D006689 Hodgkin Disease A malignant disease characterized by progressive enlargement of the lymph nodes, spleen, and general lymphoid tissue. In the classical variant, giant usually multinucleate Hodgkin's and REED-STERNBERG CELLS are present; in the nodular lymphocyte predominant variant, lymphocytic and histiocytic cells are seen. Granuloma, Hodgkin,Granuloma, Malignant,Hodgkin Lymphoma,Lymphogranuloma, Malignant,Granuloma, Hodgkin's,Granuloma, Hodgkins,Hodgkin Lymphoma, Adult,Hodgkin's Disease,Hodgkin's Lymphoma,Hodgkins Disease,Lymphocyte Depletion Hodgkin's Lymphoma,Lymphocyte-Rich Classical Hodgkin's Lymphoma,Mixed Cellularity Hodgkin's Lymphoma,Nodular Lymphocyte-Predominant Hodgkin's Lymphoma,Nodular Sclerosing Hodgkin's Lymphoma,Adult Hodgkin Lymphoma,Disease, Hodgkin,Disease, Hodgkin's,Disease, Hodgkins,Hodgkin Granuloma,Hodgkin's Granuloma,Hodgkins Granuloma,Hodgkins Lymphoma,Lymphocyte Rich Classical Hodgkin's Lymphoma,Lymphogranulomas, Malignant,Lymphoma, Hodgkin,Lymphoma, Hodgkin's,Malignant Granuloma,Malignant Granulomas,Malignant Lymphogranuloma,Malignant Lymphogranulomas,Nodular Lymphocyte Predominant Hodgkin's Lymphoma
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
t(2;5) (p23;q35)-A Specific Chromosome Abnormality in Large Cell Anaplastic (Ki-1) Lymphoma.
January 1990, Leukemia & lymphoma,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Large-cell anaplastic lymphoma-specific translocation (t[2;5] [p23;q35]) in Hodgkin's disease: indication of a common pathogenesis?
January 1995, Lancet (London, England),
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
The t(2;5)(p23;q35): a recurring chromosomal abnormality in Ki-1-positive anaplastic large cell lymphoma.
December 1989, Leukemia,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Assessment of t(2;5)(p23;q35) translocation and variants in pediatric ALK+ anaplastic large cell lymphoma.
April 2004, American journal of clinical pathology,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Detection of the t(2;5)(p23;q35) chromosomal translocation in large B-cell lymphomas other than anaplastic large cell lymphoma.
June 1996, Human pathology,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
A lymphohistiocytic variant of anaplastic large cell lymphoma with demonstration of the t(2;5)(p23;q35) chromosome translocation.
January 1998, British journal of haematology,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Diagnosis of t(2;5)(p23;q35)-associated Ki-1 lymphoma with immunohistochemistry.
December 1994, Blood,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Three children with CD30 cutaneous anaplastic large cell lymphomas bearing the t(2;5)(p23;q35) translocation.
January 2004, Pediatric dermatology,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Occurrence of the t(2;5)(p23;q35) in non-Hodgkin's lymphoma.
May 1996, Blood,
H T Yee, and M Ponzoni, and A Merson, and M Goldstein, and A Scarpa, and M Chilosi, and F Menestrina, and S Pittaluga, and C de Wolf-Peeters, and M Shiota, and S Mori, and G Frizzera, and G Inghirami
Reverse transcriptase polymerase chain reaction for the Ki-1 anaplastic large cell lymphoma-associated t(2;5) translocation in Hodgkin's disease.
December 1994, The American journal of pathology,
Copied contents to your clipboard!