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A new method of isolating the microsomal ghost fractions has been developed. The membranes were purified from adsorbed protein, ribosomes and intravesicular content. The purified membranes contained 6 times less RNA than did the original microsomes. The phospholipid:protein ratio in the ghosts increased from 0.34 to 0.80. Protein electrophoretic pattern ghost was more homogenous than that of microsomes. SDS-polyacrylamide electrophoresis showed that the ghosts contain 11 fractions as compared to 21 in microsomes. A high degree of membrane purification did not affect the inactivation of microsomal enzymic systems. Practically all cytochrome P-450 in the ghosts was found in its active form. The activities of NAD(P)H-dependent systems of dimethylaniline and ethylmorphine demethylation, p-hydroxylation of aniline were well retained. The removal of non-membrane protein allows to obtain an electrone microscope picture of the lipid bilayer as a structural basis of microsomal membrane.
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
March 1987,
The Journal of clinical investigation,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
September 1980,
Biochimica et biophysica acta,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
July 1974,
Biochimica et biophysica acta,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
July 1976,
Journal of neurochemistry,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
December 1976,
The Journal of biological chemistry,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
September 1974,
Cancer research,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
July 1976,
Journal of bacteriology,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
January 1980,
Allergologia et immunopathologia,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
May 1988,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
A I Archakov, and
G I Bachmanova, and
L V Blinder, and
V O Zhikhareva, and
I P Kanaeva
July 1974,
Archives of biochemistry and biophysics,
