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Lymphocytes isolated from the peripheral blood of patients with nonmalignant and malignant disorders were studied for fluidity of membrane lipids and lateral mobility of concanavalin A (Con A) receptors. The degree of fluidity of the surface membrane lipid core was monitored quantitatively by fluorescence polarization analysis using the probe 1,6-diphenyl-1,3,5-hexatriene embedded in lipid regions of the surface membrane of intact cells. Mobility of Con A surface receptors was determined by the cap-forming ability after binding of fluorescent Con A. The present studies were performed on lymphocytes from 28 patients with malignant lymphomas, 22 patients with leukemia, 28 individuals who either were healthy or had nonmalignant disorders, and 5 patients with carcinoma. The results showed that lymphocytes and mononuclear cells from patients with malignant lymphomas and leukemias have a more fluid lipid layer in their surface membrane than do lymphocytes obtained from healthy individuals or from patients with other malignant and nonmalignant disorders. This increase in membrane fluidity was less pronounced in lymphocytes isolated from leukemic patients in clinical remission and from leukemic patients receiving treatment with steroids. The results also show a marked difference in the cap-forming ability of lymphocytes from patients with malignant lymphomas or leukemia as compared with lymphocytes from patients with non-malignant disorders or carcinoma. Lymphocytes isolated from lymphoma and chronic lymphatic leukemia patients during remission stages of the disease exhibited a higher cap-forming ability. The cap-forming ability of cells from patients with chronic lymphocytic leukemia was unaffected by treatment with steroids. The present results, which are in line with previous observations, have shown that normal lymphocytes can be characterized by a low degree of lipid fluidity but a high degree of mobility of Con A receptors, whereas leukemic lymphocytes are characterized by a high degree of lipid fluidity but a low degree of mobility of Con A receptors. These results confirmed our general hypothesis on the dynamic interrelation between membrane lipids and membrane protein receptors, and they indicate that the widely accepted term "membrane fluidity" requires better consideration for different membrane components.
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
January 1978,
IARC scientific publications,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
March 1975,
Proceedings of the National Academy of Sciences of the United States of America,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
September 1974,
International journal of cancer,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
August 1976,
FEBS letters,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
January 1984,
Leukemia research,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
April 1976,
International journal of cancer,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
July 1979,
American journal of veterinary research,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
August 1977,
Israel journal of medical sciences,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
April 1991,
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
H Ben-Bassat, and
A Polliak, and
S M Rosenbaum, and
E Naparstek, and
D Shouval, and
M Inbar
January 1975,
Progress in medical virology. Fortschritte der medizinischen Virusforschung. Progres en virologie medicale,
