-transparent.png){kind=logo}
The metC gene coding for cystathionine beta-lyase of Escherichia coli has been cloned and used to construct an overproducing E. coli strain. An efficient purification scheme has been developed and the purified enzyme has been crystallized by the hanging drop vapour diffusion method using either ammonium sulfate or polyethyleneglycol 400 as precipitating agent. The crystals belong to the orthorombic space group C222. Their unit cell parameters are a = 60.9 A, b = 154.7 A and c = 152.7 A. Consideration of the possible values of VM accounts for the presence of one dimer per asymmetric unit. The crystals are suitable for X-ray analysis and a complete native date set to 1.83 A resolution has been collected using synchrotron radiation.
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
January 1987,
Methods in enzymology,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
December 1996,
The Biochemical journal,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
November 1995,
Plant molecular biology,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
January 2002,
Journal of bioscience and bioengineering,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
June 1991,
The Biochemical journal,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
August 1992,
Journal of bacteriology,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
February 1999,
Acta crystallographica. Section D, Biological crystallography,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
October 1994,
Protein expression and purification,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
August 2010,
Acta crystallographica. Section F, Structural biology and crystallization communications,
B Laber, and
T Clausen, and
R Huber, and
A Messerschmidt, and
U Egner, and
A Müller-Fahrnow, and
H D Pohlenz
September 1997,
FEBS letters,
