Inhibition of 1,2-dimethylhydrazine-induced oxidative DNA damage by green tea extract in rat. 1995

M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
First Department of Surgery, Kyoto Prefectural University of Medicine.

Following subcutaneous injection of 1,2-dimethylhydrazine (DMH), which is carcinogenic to rat colon and liver, to Sprague-Dawley rats, a significant increase of 8-hydroxydeoxyguanosine (8-OHdG) was observed in the DNA of colonic mucosa and liver. The 8-OHdG formation reached the maximal level at about 24 h after the DMII injection. On the other hand, no increase of 8-OHdG was observed in the DNA of the kidney. Drinking green tea extract (GTE) for ten days prior to the DMH injection significantly inhibited the formation of 8-OHdG in the colon. These findings demonstrate that DMH causes oxidative damage to the DNA of its target organ, and that GTE protects colonic mucosa from this oxidative damage.

UI MeSH Term Description Entries
D007279 Injections, Subcutaneous Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin. Subcutaneous Injections,Injection, Subcutaneous,Subcutaneous Injection
D007413 Intestinal Mucosa Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI. Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D008745 Methylation Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed) Methylations
D008746 Methylazoxymethanol Acetate The aglycone of CYCASIN. It acts as a potent carcinogen and neurotoxin and inhibits hepatic DNA, RNA, and protein synthesis. (Methyl-ONN-azoxy)methanol Acetate,Acetate, Methylazoxymethanol
D010084 Oxidation-Reduction A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471). Redox,Oxidation Reduction
D010936 Plant Extracts Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard. Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D002392 Catechin An antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms. Catechinic Acid,Catechuic Acid,(+)-Catechin,(+)-Cyanidanol,(+)-Cyanidanol-3,(-)-Epicatechin,(2R,3R)-2-(3,4-Dihydroxyphenyl)-3,5,7-chromanetriol,2H-1-Benzopyran-3,5,7-triol, 2-(3,4-dihydroxyphenyl)-3,4-dihydro-, (2R-cis)-,3,3',4',5,7-Flavanpentol,Catergen,Cianidanol,Cyanidanol-3,Epicatechin,KB-53,Z 7300,Zyma,Cyanidanol 3,KB 53,KB53

Related Publications

M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
December 2000, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
January 1989, Anticancer research,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
January 2015, Neurotoxicology and teratology,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
June 2012, Neurotoxicology,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
October 2016, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
April 2009, Pharmacological research,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
April 1996, Nihon Ika Daigaku zasshi,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
May 1988, Japanese journal of cancer research : Gann,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
June 2010, European journal of nutrition,
M Inagake, and T Yamane, and Y Kitao, and K Oya, and H Matsumoto, and N Kikuoka, and H Nakatani, and T Takahashi, and H Nishimura, and A Iwashima
December 1996, Toxicology,
Copied contents to your clipboard!