Biomaterial Database

Binding of 25-hydroxycholecalciferol in tissues. 1977

H Van Baelen, and R Bouillon, and P De Moor

Evidence is presented that the 5.8 S 25-hydroxycholecalciferol-binding protein found in the cytosols of all nucleated rat tissues is formed from two macromolecular substances: a heat-stable 4.1 S 25-hydroxycholecalciferol-binding protein which behaves identically with the serum 25-hydroxycholecalciferol-binding protein, and a cytosolic heat-labile protein which appears to sediment around 4 S and does not show binding properties for 25-hydroxycholecalciferol. The 5.8 S complex is formed in vitro by incubating cytosols with appropriate amounts of serum. The complex is dissociated by heating, leaving the serum 25-hydroxycholecalciferol-binding protein. Complex formation also occurred with serum 25-hydroxycholecalciferol-binding proteins from other species. The widespread occurrence of the 4 S cytosolic component raises the possibility that the high affinity binding proteins for 25-hydroxycholecalciferol observed in nucleated tissues are largely the result of plasma contamination.

UI	MeSH Term	Description	Entries
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols
D006887	Hydroxycholecalciferols	Hydroxy analogs of vitamin D 3; (CHOLECALCIFEROL); including CALCIFEDIOL; CALCITRIOL; and 24,25-DIHYDROXYVITAMIN D 3.	Hydroxyvitamins D,Hydroxycholecalciferol
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012260	Ribonucleases	Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.	Nucleases, RNA,RNase,Acid Ribonuclease,Alkaline Ribonuclease,Ribonuclease,RNA Nucleases,Ribonuclease, Acid,Ribonuclease, Alkaline
D014357	Trypsin	A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.	Tripcellim,Trypure,beta-Trypsin,beta Trypsin