BIOMAT Database Logo BIOMAT Database Logo

Utility of direct immunofluorescence and virus culture for detection of varicella-zoster virus in skin lesions. 1995

S E Coffin, and R L Hodinka
Department of Pediatrics, Children's Hospital of Philadelphia and School of Medicine, University of Pennsylvania 19104, USA.

A direct immunofluorescence assay (DFA) with a monoclonal antibody from Ortho Diagnostic Systems was compared with conventional cell culture for the rapid detection of varicella-zoster virus (VZV) in 140 dermal lesions from 133 patients. A total of 79 (56%) specimens were positive for VZV: 40 (51%) by DFA alone, 2 (3%) by culture only, and 37 (47%) by both culture and DFA. After discordant analysis, the sensitivities and negative predictive values, respectively, were 97.5% (77 of 79) and 96.8% (61 of 63) for DFA and 49.4% (39 of 79) and 60.4% (61 of 101) for viral culture. Of the 39 positive viral cultures, VZV was isolated from 38 (97%) cultures in A549 cells, 23 (59%) in primary rhesus monkey kidney cells, and only 16 (41%) in MRC-5 cells. We conclude that DFA is the optimal method for rapid identification of VZV. In addition, better recovery of VZV in culture may be achieved by using A549 cells.

UI MeSH Term Description Entries
D010948 Viral Plaque Assay Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE. Bacteriophage Plaque Assay,Assay, Bacteriophage Plaque,Assay, Viral Plaque,Assays, Bacteriophage Plaque,Assays, Viral Plaque,Bacteriophage Plaque Assays,Plaque Assay, Bacteriophage,Plaque Assay, Viral,Plaque Assays, Bacteriophage,Plaque Assays, Viral,Viral Plaque Assays
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D006566 Herpesviridae Infections Virus diseases caused by the HERPESVIRIDAE. Herpesvirus Infections,B Virus Infection,Infections, Herpesviridae,Infections, Herpesvirus,B Virus Infections,Herpesviridae Infection,Herpesvirus Infection,Infection, B Virus,Infection, Herpesviridae,Infection, Herpesvirus,Infections, B Virus
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D014645 Herpesvirus 3, Human The type species of VARICELLOVIRUS causing CHICKENPOX (varicella) and HERPES ZOSTER (shingles) in humans. Chickenpox Virus,Herpes zoster Virus,Ocular Herpes zoster Virus,VZ Virus,Varicella-Zoster Virus,HHV-3,Herpesvirus 3 (alpha), Human,Herpesvirus Varicellae,Human Herpesvirus 3,Chickenpox Viruses,Herpes zoster Viruses,VZ Viruses,Varicella Zoster Virus,Varicella-Zoster Viruses,Varicellae, Herpesvirus
D017193 Skin Diseases, Viral Skin diseases caused by viruses. Viral Skin Diseases,Disease, Viral Skin,Diseases, Viral Skin,Skin Disease, Viral,Viral Skin Disease
D019085 Fluorescent Antibody Technique, Direct A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984) Immunofluorescence Antibody Test, Direct,Immunofluorescence Technique, Direct,Direct Fluorescent Antibody Technic,Direct Fluorescent Antibody Technique,Direct Immunofluorescence,Direct Immunofluorescence Assay,Fluorescent Antibody Technic, Direct,Immunofluorescence Technic, Direct,Assay, Direct Immunofluorescence,Assays, Direct Immunofluorescence,Direct Immunofluorescence Assays,Direct Immunofluorescence Technic,Direct Immunofluorescence Technics,Direct Immunofluorescence Technique,Direct Immunofluorescence Techniques,Direct Immunofluorescences,Immunofluorescence Assay, Direct,Immunofluorescence Assays, Direct,Immunofluorescence Technics, Direct,Immunofluorescence Techniques, Direct,Immunofluorescence, Direct,Immunofluorescences, Direct

Related Publications

S E Coffin, and R L Hodinka
Comparison of virus culture and direct immunofluorescent staining of cytocentrifuged virus transport medium for detection of varicella-zoster virus in skin lesions.
May 1998, American journal of clinical pathology,
S E Coffin, and R L Hodinka
Direct immunofluorescence staining for detection of herpes simplex and varicella-zoster virus antigens in vesicular lesions and certain tissue specimens.
November 1980, Journal of clinical microbiology,
S E Coffin, and R L Hodinka
Should varicella-zoster virus culture be eliminated? A comparison of direct immunofluorescence antigen detection, culture, and PCR, with a historical review.
December 2012, Journal of clinical microbiology,
S E Coffin, and R L Hodinka
Rapid diagnosis of varicella-zoster virus infection by direct immunofluorescence.
May 1980, American journal of clinical pathology,
S E Coffin, and R L Hodinka
Detection of herpes simplex and varicella zoster viruses in clinical specimens using direct immunofluorescence and cell culture assays.
January 2001, Microbios,
S E Coffin, and R L Hodinka
Replication of varicella-zoster virus in human skin organ culture.
September 2005, Journal of virology,
S E Coffin, and R L Hodinka
Detection of cutaneous varicella zoster virus infections by immunofluorescence versus PCR.
January 2001, European journal of dermatology : EJD,
S E Coffin, and R L Hodinka
Comparison of Chemicon SimulFluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence staining for detection of varicella-zoster virus.
September 2001, Clinical and diagnostic laboratory immunology,
S E Coffin, and R L Hodinka
[Varicella-zoster virus-associated spinal myoclonus without skin lesions].
April 1992, Rinsho shinkeigaku = Clinical neurology,
S E Coffin, and R L Hodinka
Comparison of detection of varicella-zoster virus by the Tzanck smear, direct immunofluorescence with a monoclonal antibody, and virus isolation.
July 1987, Journal of the American Academy of Dermatology,
Copied contents to your clipboard!