Biomaterial Database

Cytogenetic and genetic studies of radiation-induced chromosome damage in mouse oocytes. II. Induced chromosome loss and dominant visible mutations. 1996

C Tease, and G Fisher

MRC Radiobiology Unit, Chilton, Didcot, UK.

The rates of X-ray induced loss of chromosome 19 in mouse oocytes were investigated in 2 experiments using a genetic complementation test. After 1 Gy of acute X-rays to immediately preovulatory stage oocytes, chromosome 19 loss was estimated to have occurred in 1.68% of cells. In comparison, after 4 Gy of acute X-rays to dictyate stage oocytes, the rate was estimated at 1.18%. The slightly higher rate of chromosome loss in the former cell stage after a smaller dose of radiation reflects the known increased radiosensitivity of mouse oocytes in the period shortly before ovulation. Comparison of the observations here for chromosome 19 with published data for chromosome 1 suggests that chromosome length is one of the principal factors in determining the initial rate of induced loss in mouse oocytes. Ten dominant visible mutations were recovered among 1674 offspring following irradiation of preovulatory oocytes, and 8 in 2025 offspring after treatment of dictyate cells. Nine dominant mutations were karyotyped, 5 of these were found to be associated with a visible chromosome rearrangement. The data obtained in the present study show that radiation-induced chromosome anomalies in female germ cells are not all filtered out by prenatal embryonic death but that a proportion has the potential to contribute to the genetic burden of the next generation.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008809	Mice, Inbred C3H	An inbred strain of mouse that is used as a general purpose strain in a wide variety of RESEARCH areas including CANCER; INFECTIOUS DISEASES; sensorineural, and cardiovascular biology research.	Mice, C3H,Mouse, C3H,Mouse, Inbred C3H,C3H Mice,C3H Mice, Inbred,C3H Mouse,C3H Mouse, Inbred,Inbred C3H Mice,Inbred C3H Mouse
D009865	Oocytes	Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).	Ovocytes,Oocyte,Ovocyte
D002872	Chromosome Deletion	Actual loss of portion of a chromosome.	Monosomy, Partial,Partial Monosomy,Deletion, Chromosome,Deletions, Chromosome,Monosomies, Partial,Partial Monosomies
D004307	Dose-Response Relationship, Radiation	The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation.	Dose Response Relationship, Radiation,Dose-Response Relationships, Radiation,Radiation Dose-Response Relationship,Radiation Dose-Response Relationships,Relationship, Radiation Dose-Response,Relationships, Radiation Dose-Response
D005260	Female		Females
D005799	Genes, Dominant	Genes that influence the PHENOTYPE both in the homozygous and the heterozygous state.	Conditions, Dominant Genetic,Dominant Genetic Conditions,Genetic Conditions, Dominant,Condition, Dominant Genetic,Dominant Gene,Dominant Genes,Dominant Genetic Condition,Gene, Dominant,Genetic Condition, Dominant
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus