Biomaterial Database

Improved glutaminate-starch-penicillin agar for the isolation and enumeration of Aeromonas hydrophila from seawater by membrane filtration. 1995

M Papapetropoulou, and G Rodopoulou, and E Giannoulaki

University of Patras, Medical School, Laboratory of Health Sciences, Greece.

Glutaminate-starch-penicillin agar (GSP) in its original formula was found unsatisfactory for the detection and the enumeration of Aeromonas hydrophila from seawater by membrane filtration. It was also the case when GSP agar was adjusted to pH 8 or when ampicillin (10 mg/L) was incorporated to the original formula. The elevation of pH to 8 together with the addition of 10 mg/L ampicillin gave to the medium a high selectivity and permitted the detection of A. hydrophila from seawater. The numbers of A. hydrophila were monitored at 11 beaches along the coastline of Patras and the distribution of Aeromonas was not found homogenous. Low numbers of A. hydrophila were isolated from most samples tested, whereas high concentrations of A. hydrophila were isolated specifically from samples that were collected near sewage and fresh water inputs.

UI	MeSH Term	Description	Entries
D009792	Oceans and Seas	A great expanse of continuous bodies of salt water which together cover more than 70 percent of the earth's surface. Seas may be partially or entirely enclosed by land, and are smaller than the five oceans (Atlantic, Pacific, Indian, Arctic, and Antarctic).	Seas,Oceans,Seas and Oceans
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D000667	Ampicillin	Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.	Penicillin, Aminobenzyl,Amcill,Aminobenzylpenicillin,Ampicillin Sodium,Ampicillin Trihydrate,Antibiotic KS-R1,Omnipen,Pentrexyl,Polycillin,Ukapen,Aminobenzyl Penicillin,Antibiotic KS R1,KS-R1, Antibiotic,Sodium, Ampicillin,Trihydrate, Ampicillin
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D014871	Water Microbiology	The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.	Microbiology, Water
D016980	Aeromonas hydrophila	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that may be pathogenic for frogs, fish, and mammals, including man. In humans, cellulitis and diarrhea can result from infection with this organism.
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In