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OBJECTIVE To study the effect of retinoic acid on the expression of transforming growth factor-beta (TGF-beta) by human retinal pigment epithelial (RPE) cells in culture. METHODS Expression of TGF-beta 1 by RPE cells after 24 hours in culture at the messenger RNA level (Northern analysis) and the protein level (enzyme-linked immunosorbent assay [ELISA]). RESULTS On the basis of cell morphology, growth characteristics and the results of immunohistochemical studies, we concluded that the cells used in these experiments were RPE in origin. Immunohistochemical studies confirmed TGF-beta 1 expression by the RPE cells in culture. Densitometry showed that retinoic acid reduced the level of TGF-beta 1 mRNA expression by 41% compared with control samples. ELISA showed that retinoic acid inhibited TGF-beta expression when compared with the baseline level of TGF-beta in media from RPE cells in culture. CONCLUSIONS These findings may have implications for the pharmacotherapy of proliferative vitreoretinopathy.
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
C Peters, and
E E Tredget, and
A Ghahary
January 1999,
Ophthalmic research,
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
C Peters, and
E E Tredget, and
A Ghahary
May 1994,
Journal of cellular physiology,
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
C Peters, and
E E Tredget, and
A Ghahary
January 1994,
Ophthalmic research,
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
C Peters, and
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A Ghahary
January 2008,
Molecular vision,
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
C Peters, and
E E Tredget, and
A Ghahary
February 1993,
Investigative ophthalmology & visual science,
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
C Peters, and
E E Tredget, and
A Ghahary
December 1994,
Investigative ophthalmology & visual science,
I M MacDonald, and
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K Kovithavongs, and
C Peters, and
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A Ghahary
December 2003,
Journal of cellular physiology,
I M MacDonald, and
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K Kovithavongs, and
C Peters, and
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March 1995,
Investigative ophthalmology & visual science,
I M MacDonald, and
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K Kovithavongs, and
C Peters, and
E E Tredget, and
A Ghahary
June 2017,
Cell biochemistry and function,
I M MacDonald, and
R Pannu, and
K Kovithavongs, and
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E E Tredget, and
A Ghahary
January 1996,
Life sciences,
