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Many sets of genes in Saccharomyces cerevisiae are divergently transcribed, but at present there are no vectors generally available for the simultaneous analysis of divergent transcription from these promoters. In the present study MEL1 and lacZ were used to construct a vector capable of measuring the divergent expression initiated by the MAL6T-MAL6S bi-directional promoter. Our observations demonstrate that the expression of both reporter genes was regulated in a similar fashion to the native MAL6T and MAL6S genes, and that induction was dependent upon the presence of a functional MALR activator gene. The results confirmed that the MAL6T-MAL6S promoter was co-ordinately regulated, repressed by glucose, induced by maltose, and that basal expression was more active in the MAL6S direction than in the MAL6T direction.
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
September 1994,
Molecular & general genetics : MGG,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
February 2002,
Molecular microbiology,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
February 1995,
Gene,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
June 1993,
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P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
September 1990,
Molecular & general genetics : MGG,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
January 1991,
Journal of bacteriology,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
August 1984,
Molecular and cellular biology,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
January 2002,
FEMS microbiology letters,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
January 1997,
The Journal of eukaryotic microbiology,
P J Bell, and
P H Bissinger, and
R J Evans, and
I W Dawes
February 1996,
Applied microbiology and biotechnology,
