Biomaterial Database

Multifunctional proteins in Tetrahymena: 14-nm filament protein/citrate synthase and translation elongation factor-1 alpha. 1996

O Numata

Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.

One gene encoding a protein has been shown to have two entirely different functions. Such a phenomenon, which has been called "gene sharing," was first known in crystallins. We found two multifunctional proteins in the ciliated protozoan Tetrahymena: 14-nm filament protein and protein translation elongation factor 1-alpha (EF-1 alpha). The 14-nm filament protein has dual functions as a citrate synthase in mitochondria and as a cytoskeletal protein in cytoplasm. In cytoplasm, the 14-nm filament protein was involved in oral morphogenesis and in pronuclear behavior during conjugation. The observation that Tetrahymena intramitochondrial filamentous inclusions contain the 14-nm filament protein and that the citrate synthase activity of the 14-nm filament protein is decreased by polymerization and increased by depolymerization, suggests a possible modulating mechanism of citrate synthase activity by monomer-polymer conversion in mitochondria in situ. The EF-1 alpha functions as an F-actin-bundling protein and a 14-nm filament-associated protein as well as an elongation factor in protein synthesis. The F-actin-bundling activity of EF-1 alpha was regulated by Ca2+ and calmodulin. Here we review the properties and functions of two multifunctional proteins in Tetrahymena.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010445	Peptide Elongation Factors	Protein factors uniquely required during the elongation phase of protein synthesis.	Elongation Factor,Elongation Factors, Peptide,Factor, Elongation,Factors, Peptide Elongation
D002950	Citrate (si)-Synthase	Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.	Citrate Synthase,Synthase, Citrate
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013768	Tetrahymena	A genus of ciliate protozoa commonly used in genetic, cytological, and other research.	Tetrahymenas
D015800	Protozoan Proteins	Proteins found in any species of protozoan.	Proteins, Protozoan
D020648	Peptide Elongation Factor 1	Peptide elongation factor 1 is a multisubunit protein that is responsible for the GTP-dependent binding of aminoacyl-tRNAs to eukaryotic ribosomes. The alpha subunit (EF-1alpha) binds aminoacyl-tRNA and transfers it to the ribosome in a process linked to GTP hydrolysis. The beta and delta subunits (EF-1beta, EF-1delta) are involved in exchanging GDP for GTP. The gamma subunit (EF-1gamma) is a structural component.	EF-1,Elongation Factor 1,Elongation Factor 1alpha,Elongation Factor 1beta,Elongation Factor 1delta,Elongation Factor 1gamma,EF 1,EF-1H,EF-1alpha,EF-1beta,EF-1delta,EF-1gamma,EF 1H,EF 1alpha,EF 1beta,EF 1delta,EF 1gamma