Biomaterial Database

SYBR green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. 1995

F Karlsen, and H B Steen, and J M Nesland

Department of Pathology, Norwegian Cancer Society, Oslo, Norway.

UI	MeSH Term	Description	Entries
D009930	Organic Chemicals	A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.	Organic Chemical,Chemical, Organic,Chemicals, Organic
D011804	Quinolines
D003959	Diamines	Organic chemicals which have two amino groups in an aliphatic chain.	Diamine
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D004996	Ethidium	A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.	Ethidium Bromide,Homidium Bromide,Novidium,Bromide, Ethidium,Bromide, Homidium
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D014407	Tumor Cells, Cultured	Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.	Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

Related Publications

F Karlsen, and H B Steen, and J M Nesland

[Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction].

June 2002, Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,

F Karlsen, and H B Steen, and J M Nesland

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.

February 2021, 3 Biotech,

F Karlsen, and H B Steen, and J M Nesland

Detection and quantification of hepatitis B virus DNA by SYBR green real-time polymerase chain reaction.

January 2007, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,

F Karlsen, and H B Steen, and J M Nesland

Detection of adenovirus DNA in clinical samples by SYBR Green real-time polymerase chain reaction assay.

June 2005, Pediatrics international : official journal of the Japan Pediatric Society,

F Karlsen, and H B Steen, and J M Nesland

Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction.

January 2004, Acta virologica,

F Karlsen, and H B Steen, and J M Nesland

[SYBR green I real-time polymerase chain reaction for detection of Norovirus II in the shellfish].

June 2010, Sheng wu gong cheng xue bao = Chinese journal of biotechnology,

F Karlsen, and H B Steen, and J M Nesland

Detection of coralyne and heparin by polymerase extension reaction using SYBR Green I.

August 2019, Molecular and cellular probes,