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Using the Sephadex Gel-filtration method (Steeno - 1975) we carried out a review of the data on the possibility of enriching X-spermatozoa. The testing procedure followed consisted of determining Y-chromatin positive spermatozoa after staining with quinacrine-mustard. We were able to confirm the data recorded by Steeno, according to which an enrichment of about 80-90% can be achieved after penetration through the Sephadex Gel filter. In addition to these investigations we also carried out tests with the enriched X-spermatozoa material to determine in vitro penetration through cervical mucus. No decline in the progressive motility of the spermatozoa was observed. After the Gel-filtration sufficient numbers of these were still able to cover penetration distances of 60 mm/hour. The enrichment effect of the X-spermatozoa declined at the beginning of in vitro penetration, but there was no further change in the subsequent course of penetration.
K H Broer, and
U Dauber, and
R Kaiser
January 1963,
Fertility and sterility,
K H Broer, and
U Dauber, and
R Kaiser
June 1980,
Fertility and sterility,
K H Broer, and
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R Kaiser
July 1987,
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K H Broer, and
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R Kaiser
January 1989,
Symposia of the Society for Experimental Biology,
K H Broer, and
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R Kaiser
January 1983,
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K H Broer, and
U Dauber, and
R Kaiser
August 1954,
The Journal of obstetrics and gynaecology of the British Empire,
K H Broer, and
U Dauber, and
R Kaiser
June 1982,
Fertility and sterility,
K H Broer, and
U Dauber, and
R Kaiser
November 1948,
Lancet (London, England),
K H Broer, and
U Dauber, and
R Kaiser
January 1973,
Acta obstetricia et gynecologica Scandinavica,
K H Broer, and
U Dauber, and
R Kaiser
March 1986,
Fertility and sterility,
