-transparent.png){kind=logo}
It is the second peptidase inhibitor, after ovostatin, which showing the same antipapain activity in egg white in different avian species implies differences in amino-acid sequences. Cystatin from duck egg white was purified by carboxymethylpapain affinity chromatography and size-exclusion HPLC. The purified inhibitor which showed partial identity in the immunodiffusion test with chicken egg white cystatin, had an apparent molecular mass of 9.3 kDa as determined by SDS/PAGE. IEF analysis revealed five molecular forms of pI in the range 7.8-8.4. The obtained cystatin was neither glycosylated nor phosphorylated as it is in the case of chicken cystatin. The determined Ki (0.005 +/- 0.001 nM) was similar to that reported for human and chicken cystatin C.
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
October 2014,
Biochemical and biophysical research communications,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
November 2022,
Foods (Basel, Switzerland),
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
April 2020,
Food chemistry,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
April 1983,
The Biochemical journal,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
January 1967,
Biochemical and biophysical research communications,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
January 2021,
Food & function,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
June 1980,
Biochimica et biophysica acta,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
May 1958,
The Indian journal of medical research,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
December 2015,
Food chemistry,
M Warwas, and
J Gburek, and
J Osada, and
K Gołab
January 1971,
The Journal of biological chemistry,
