-transparent.png){kind=logo}
Microtubule inhibitors are active against the human malarial parasite Plasmodium falciparum, but whether these drugs actually interact with parasite tubulins is not known. It has not previously been possible to produce mg quantities of isolated, soluble tubulin subunits for drug-binding experiments. A cDNA encoding P falciparum beta-tubulin was expressed and the protein secreted in Bacillus brevis. With the addition of EGTA to the culture medium, which increases shedding of proteins from the cell surface, up to 2 mg/l recombinant beta-tubulin was obtained in supernatants. It is not clear why B brevis is able to secrete this normally cytoplasmic protein, but the secretion levels of recombinant proteins may be related to the net charge of the first few residues of the mature polypeptide.
A Bell, and
B Wernli, and
R M Franklin
November 1989,
Gene,
A Bell, and
B Wernli, and
R M Franklin
August 2005,
FEMS immunology and medical microbiology,
A Bell, and
B Wernli, and
R M Franklin
January 1993,
Antonie van Leeuwenhoek,
A Bell, and
B Wernli, and
R M Franklin
January 1995,
Advances in experimental medicine and biology,
A Bell, and
B Wernli, and
R M Franklin
February 1992,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
A Bell, and
B Wernli, and
R M Franklin
December 1996,
Vaccine,
A Bell, and
B Wernli, and
R M Franklin
January 2004,
Bioscience, biotechnology, and biochemistry,
A Bell, and
B Wernli, and
R M Franklin
January 1994,
Bioprocess technology,
A Bell, and
B Wernli, and
R M Franklin
January 1993,
Methods in enzymology,
A Bell, and
B Wernli, and
R M Franklin
January 1992,
Bioscience, biotechnology, and biochemistry,
