-transparent.png){kind=logo}
The relationship between bone marrow (BM) cells with S-phase DNA content and the amount of peripheral blood contamination estimated as percentage lymphocytes+monocytes (L+MO) present in BM samples has been investigated in a total of 136 BM aspirates and biopsy expellates from 35 hematologically healthy individuals. A significant negative correlation was demonstrated between total, erythroid and myeloid BM cells in S-phase and the percentage of L+MO in the aspirates (r=0.84, 0.57 and 0.49, respectively; p<0.0001). Based on the equation of the slope of the regression line, a correction formula adjusting the measured value of BM cells in S-phase to varying amounts of L+MO percentage has been worked out for the total and erythroid BM cells. In contrast, highly proliferating myelomonocytic cells and CD34+ cells did not show any significant correlation between cells in S-phase and percentage L+MO, indicating that peripheral blood contamination of BM aspirates estimates the degree of peripheral blood contamination, as well as make possible a correct estimation of the DNA synthesis of several BM populations. The method is especially applicable when frequent BM sampling is required.
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
January 2009,
Cytometry. Part B, Clinical cytometry,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
January 2010,
Pediatric blood & cancer,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
November 1997,
British journal of haematology,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
January 1994,
Blood,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
February 1998,
Cancer,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
January 2019,
Methods in molecular biology (Clifton, N.J.),
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
May 2006,
Analytical biochemistry,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
April 2000,
The veterinary quarterly,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
July 2004,
American journal of clinical pathology,
J F Abrahamsen, and
R Smaaland, and
R Skjaerven, and
O D Laerum
March 1991,
Journal of immunological methods,
