Image analysis DNA densitometry measurements on complete and partial hydatidiform mole and nonmolar products of conception. 1995

R A Williams, and I G Charlton, and A J Howat
Wangaratta and District Pathology Service, Victoria, Australia.

Most spontaneous first trimester abortions have an abnormal karyotype, some being triploid; the latter are said to show characteristic histological changes, including focal villous hydropic change, focal trophoblast hyperplasia, cisternal cavitation of larger villi, irregular outline to villi, and trophoblast inclusions. To date, DNA-ploidy profiles have been derived from flow cytometry and cytogenetic karyotyping, both methods being time-consuming and expensive. This study has assessed DNA ploidy on products of conception using a simple video image analysis system. It is concluded that diploid, triploid, aneuploid, and hyperdiploid states can be ascertained on archival paraffin-embedded material using interactive image analysis morphometry. However, the stated histological criteria for triploidy (which has previously been equated with partial mole) appear to be unreliable, with only three of eight histological partial moles having this DNA pattern. In addition, there were six diploid, five triploid, seven hyperdiploid, and two aneuploid DNA ploidy profiles in the spontaneous nonmolar pregnancies. We found that of the 11 complete moles, five were aneuploid and six were hyperdiploid, with none being either diploid or triploid. This finding is contrary to the commonly held view that all complete moles have a diploid DNA profile.

UI MeSH Term Description Entries
D011003 Ploidies The degree of replication of the chromosome set in the karyotype. Ploidy
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D004273 DNA, Neoplasm DNA present in neoplastic tissue. Neoplasm DNA
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006828 Hydatidiform Mole Trophoblastic hyperplasia associated with normal gestation, or molar pregnancy. It is characterized by the swelling of the CHORIONIC VILLI and elevated human CHORIONIC GONADOTROPIN. Hydatidiform moles or molar pregnancy may be categorized as complete or partial based on their gross morphology, histopathology, and karyotype. Molar Pregnancy,Pregnancy, Molar,Hydatid Mole,Hydatidiform Mole, Complete,Hydatidiform Mole, Partial,Complete Hydatidiform Mole,Complete Hydatidiform Moles,Hydatid Moles,Hydatidiform Moles,Hydatidiform Moles, Complete,Hydatidiform Moles, Partial,Molar Pregnancies,Mole, Complete Hydatidiform,Mole, Hydatid,Mole, Hydatidiform,Mole, Partial Hydatidiform,Moles, Complete Hydatidiform,Moles, Hydatid,Moles, Hydatidiform,Moles, Partial Hydatidiform,Partial Hydatidiform Mole,Partial Hydatidiform Moles,Pregnancies, Molar
D000030 Abortion, Missed The retention in the UTERUS of a dead FETUS two months or more after its DEATH. Abortions, Missed,Missed Abortion,Missed Abortions
D014594 Uterine Neoplasms Tumors or cancer of the UTERUS. Cancer of Uterus,Uterine Cancer,Cancer of the Uterus,Neoplasms, Uterine,Neoplasms, Uterus,Uterus Cancer,Uterus Neoplasms,Cancer, Uterine,Cancer, Uterus,Cancers, Uterine,Cancers, Uterus,Neoplasm, Uterine,Neoplasm, Uterus,Uterine Cancers,Uterine Neoplasm,Uterus Cancers,Uterus Neoplasm
D019044 Image Cytometry A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining. Cytometry, Image,Cytometries, Image,Image Cytometries

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