OBJECTIVE To determine the pulpal biocompatibility of Prisma Universal Bond 2 (PUB-2). METHODS Class V preparations were made in eight subhuman monkeys to approximately 1 mm from the pulp. In Group 1, the enamel was etched, the primer was placed and dried, PUB 2 was painted, and Prisma-Fil inserted. In Goup 2, the cavity floor was covered with visible light-cured Dycal followed by the steps as in group 1. In Group 3, IRM was used as a negative control. In Group 4, MQ55 silicate cement was used as a positive control. The animals were sacrificed at 5, 25, and 60 days and the teeth processed for histological evaluation. RESULTS After 5 days, a mild reaction of little concern, which had dissipated in the 25- and 6-day groups, was observed. The material compared favorably to the negative control.