Biomaterial Database

Diastereomeric specificity of 2',3'-cyclic nucleotide 3'-phosphodiesterase. 1996

P A Heaton, and F Eckstein

Max-Planck-Institut fur experimentelle Medizin, Göttingen, Germany.

The diastereomers of adenosine and uridine 2',3'-cyclic phosphorothioates were tested as substrates for 2',3'-cyclic nucleotide 3'-phosphodiesterase from bovine brain. The enzyme cleaves the Sp (or exo) diastereomers efficiently, whereas the Rp (or endo) diastereomers are resistant to hydrolysis, even after long incubation. As the enzyme exhibits strong substrate inhibition the precise determination of kinetic parameters posed problems, particularly with phosphorothioates. The stereoselectivity of this enzyme is opposite to that of RNase T1 and RNase A and thus could be a useful complement in determination of the configuration of nucleoside 2',3'-cyclic phosphorothioates resulting from hydrolysis reactions of unknown stereochemical course.

UI	MeSH Term	Description	Entries
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013237	Stereoisomerism	The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)	Molecular Stereochemistry,Stereoisomers,Stereochemistry, Molecular,Stereoisomer
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D015087	2',3'-Cyclic-Nucleotide Phosphodiesterases	Nucleoside-2',3'-cyclic phosphate nucleotidohydrolase. Enzymes that catalyze the hydrolysis of the 2'- or 3'- phosphate bonds of 2',3'-cyclic nucleotides. Also hydrolyzes nucleoside monophosphates. Includes EC 3.1.4.16 and EC 3.1.4.37. EC 3.1.4.-.	2',3'-Cyclic Nucleotide Phosphodiesterase,2',3'-Cyclic Nucleotide Phosphodiesterases,2,3-Cyclic Nucleotide Phosphodiesterase,2,3-Cyclic Nucleotide Phosphodiesterases,CNPase,2',3' Cyclic Nucleotide Phosphodiesterase,2',3' Cyclic Nucleotide Phosphodiesterases,2,3 Cyclic Nucleotide Phosphodiesterase,2,3 Cyclic Nucleotide Phosphodiesterases,Nucleotide Phosphodiesterase, 2',3'-Cyclic,Nucleotide Phosphodiesterase, 2,3-Cyclic,Nucleotide Phosphodiesterases, 2',3'-Cyclic,Nucleotide Phosphodiesterases, 2,3-Cyclic,Phosphodiesterase, 2',3'-Cyclic Nucleotide,Phosphodiesterase, 2,3-Cyclic Nucleotide,Phosphodiesterases, 2',3'-Cyclic Nucleotide,Phosphodiesterases, 2',3'-Cyclic-Nucleotide,Phosphodiesterases, 2,3-Cyclic Nucleotide