Increased epithelial cell proliferation in nasal polyps. 1996

A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
Department of Oto-Rhino-Laryngology and Head and Neck Surgery, Henri Mondor and Interncommunal University Hospitals, University of Paris XII, Creteil, France.

OBJECTIVE To detect, quantify, and compare respiratory epithelial cell proliferation in nasal mucosa and polyps from patients with nasal polyposis. METHODS Cohort study. METHODS Patients and samples were selected at the Hôpital Intercommunal de Créteil (France). Flow cytofluorometry and immunohistochemistry were performed at Hôpitaux Tenon and Mondor (Université Paris [France] VI et XII). METHODS Twenty-one patients undergoing endoscopic ethmoidectomy for treatment of nasal polyposis. METHODS In 10 cases, epithelial cells were removed from frozen inferior turbinate mucosa and polyps by mechanical disaggregation and were then analyzed by flow cytofluorometry, providing the cell DNA content (propidium iodide labeling) and the percentage of S-phase cells. In 11 cases, inferior turbinate mucosa and polyps were fixed in formaldehyde and embedded in paraffin. Proliferating cell nuclear antigen expression in the epithelium was quantified by immunohistochemistry; a proliferating cell nuclear antigen index was calculated for each sample in the basal area, suprabasal area, and full height of the epithelium. RESULTS All cell populations studied were diploid, and percentages of S-phrase cells were significantly higher in nasal polyps than in mucosa. Proliferating cell nuclear antigen indexes were significantly higher in nasal polyps than in the suprabasal area and full height of the mucosal epithelium. CONCLUSIONS Cell proliferation is increased in epithelium from nasal polyps. Epithelial damage caused by inflammatory mediators could induce this increased cell proliferation via epithelial repair processes. Inflammatory cells could up-regulate epithelial cell proliferation by secreting growth factors.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008940 Mitotic Index An expression of the number of mitoses found in a stated number of cells. Index, Mitotic,Indices, Mitotic,Mitotic Indices
D009297 Nasal Mucosa The mucous lining of the NASAL CAVITY, including lining of the nostril (vestibule) and the OLFACTORY MUCOSA. Nasal mucosa consists of ciliated cells, GOBLET CELLS, brush cells, small granule cells, basal cells (STEM CELLS) and glands containing both mucous and serous cells. Nasal Epithelium,Schneiderian Membrane,Epithelium, Nasal,Membrane, Schneiderian,Mucosa, Nasal
D009298 Nasal Polyps Focal accumulations of EDEMA fluid in the NASAL MUCOSA accompanied by HYPERPLASIA of the associated submucosal connective tissue. Polyps may be NEOPLASMS, foci of INFLAMMATION, degenerative lesions, or malformations. Nasal Polyp,Polyp, Nasal,Polyps, Nasal
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D004171 Diploidy The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X. Diploid,Diploid Cell,Cell, Diploid,Cells, Diploid,Diploid Cells,Diploidies,Diploids
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell

Related Publications

A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
March 2002, Journal of the Formosan Medical Association = Taiwan yi zhi,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
January 2005, Otolaryngologia polska = The Polish otolaryngology,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
September 2022, American journal of rhinology & allergy,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
January 2015, PloS one,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
October 2023, bioRxiv : the preprint server for biology,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
February 2023, Clinical immunology (Orlando, Fla.),
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
May 1996, The Laryngoscope,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
May 1984, Clinical allergy,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
April 2014, Scientific reports,
A Coste, and J G Rateau, and F Roudot-Thoraval, and C Chapelin, and L Gilain, and F Poron, and R Peynegre, and J F Bernaudin, and E Escudier
January 1978, The Annals of otology, rhinology, and laryngology,
Copied contents to your clipboard!