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Identification of an 85 kDa phosphoprotein as an immunodominant protein specific for human herpesvirus 7-infected cells. 1996

L Foà-Tomasi, and M P Fiorilli, and E Avitabile, and G Campadelli-Fiume
Department of Experimental Pathology, Section of Microbiology and Virology, University of Bologna, Italy.

The reactivity of human cord blood sera was directed most frequently in Western blot assays to a protein with an apparent molecular mass of 85 kDa that belongs to the p85 complex, a family of antigenically related proteins identified previously in our laboratory with the aid of two MAbs. We show that the 85 kDa protein is phosphorylated. As antibodies present in the human sera were directed in part to proteins carrying cross-reactive epitopes between human herpesvirus 6 (HHV-6) and 7 (HHV-7), it is remarkable that reactivity to the 85 kDa phosphoprotein was maintained after preabsorption of the sera with HHV-6 antigen, but abolished after preabsorption with HHV-7 antigen. Therefore, the 85 kDa phosphoprotein may be considered a major determinant of the human immune response to HHV-7, discriminating HHV-6 from HHV-7 infection.

UI MeSH Term Description Entries
D010750 Phosphoproteins Phosphoprotein
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D005312 Fetal Blood Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery. Cord Blood,Umbilical Cord Blood,Blood, Cord,Blood, Fetal,Blood, Umbilical Cord,Bloods, Cord,Bloods, Fetal,Bloods, Umbilical Cord,Cord Blood, Umbilical,Cord Bloods,Cord Bloods, Umbilical,Fetal Bloods,Umbilical Cord Bloods
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D015654 Herpesvirus 6, Human Members of the ROSEOLOVIRUS genus of the Betaherpesvirales subfamily isolated from patients with AIDS and other LYMPHOPROLIFERATIVE DISORDERS. It infects and replicates in fresh and established lines of hematopoietic cells and cells of neural origin. It also appears to alter the activity of NK CELLS. HHV-6; (HBLV) antibodies are elevated in patients with AIDS; SJOGREN'S SYNDROME; SARCOIDOSIS; CHRONIC FATIGUE SYNDROME, and certain malignancies. HHV-6A is the most common cause of EXANTHEMA SUBITUM and has been implicated in encephalitis. When HHV-6 integrates into the host genome it is referred to as ciHVH-6. When such VIRUS INTEGRATION occurs into the germline it is referred to as iciHHV-6. HBLV,Herpesvirus 6A, Human,Herpesvirus 6B, Human,Human B-Lymphotropic Virus,Chromosomally Integrated Human Herpesvirus 6,Chromosomally Integrated Human Herpesvirus 6A,Chromosomally Integrated Human Herpesvirus 6B,HHV-6,HHV-6A,HHV-6B,HHV6,HHV6A,HHV6B,Human Herpesvirus 6,Human betaherpesvirus 6,Human betaherpesvirus 6A,Human betaherpesvirus 6B,Inherited Chromosomally Integrated Human Herpesvirus 6,Inherited Chromosomally Integrated Human Herpesvirus 6A,Inherited Chromosomally Integrated Human Herpesvirus 6B,ciHHV-6,ciHHV-6A,ciHHV-6B,ciHHV6,ciHHV6A,ciHHV6B,iciHHV-6,iciHHV-6A,iciHHV-6B,iciHHV6,iciHHV6A,iciHHV6B,B-Lymphotropic Virus, Human,B-Lymphotropic Viruses, Human,Human B Lymphotropic Virus,Human B-Lymphotropic Viruses,Human Herpesvirus 6A,Human Herpesvirus 6B,Human betaherpesvirus 6s
D016056 Immunodominant Epitopes Subunits of the antigenic determinant that are most easily recognized by the immune system and thus most influence the specificity of the induced antibody. Antigenic Determinants, Immunodominant,Antigens, Immunodominant,Epitopes, Immunodominant,Immunodominant Determinant,Immunodominant Domain,Immunodominant Epitope,Immunodominant Region,Immunodominant Site,Immunodominant Determinants,Immunodominant Domains,Immunodominant Regions,Immunodominant Sites,Determinant, Immunodominant,Determinants, Immunodominant,Determinants, Immunodominant Antigenic,Domain, Immunodominant,Domains, Immunodominant,Epitope, Immunodominant,Immunodominant Antigenic Determinants,Immunodominant Antigens,Region, Immunodominant,Regions, Immunodominant,Site, Immunodominant,Sites, Immunodominant
D016199 Herpesvirus 7, Human A species in the genus ROSEOLOVIRUS, of the family HERPESVIRIDAE. It was isolated from activated, CD4-positive T-lymphocytes taken from the blood of a healthy human. HHV-7,Human Herpesvirus 7

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