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The influence of experimental errors on the determination of flux control coefficients from transient metabolite concentrations with the method proposed by Delgado and Liao [(1992) Biochem. J. 282, 919-927] has been investigated by using Monte Carlo simulations. The method requires least-squares fitting of the transient metabolite concentrations. Three different fitting methods have been evaluated. Simulated metabolite concentrations of a fictive metabolic pathway were scattered randomly, emulating experimental errors, before performing the fits. This was repeated a large number of times; the mean values and standard deviations of the resulting control coefficients are reported. The results show that the proposed method for determining control coefficients is too sensitive to experimental errors to be practicable, with theoretically justified fitting methods. This is in particular due to the high degree of correlation between the concentrations. An alternative ad hoc fitting method produced biased mean values of the estimates of the control coefficients, but with remarkably low standard deviations.
M Ehlde, and
G Zacchi
March 1992,
The Biochemical journal,
M Ehlde, and
G Zacchi
August 1992,
The Biochemical journal,
M Ehlde, and
G Zacchi
March 2005,
Metabolic engineering,
M Ehlde, and
G Zacchi
May 1993,
Biotechnology and bioengineering,
M Ehlde, and
G Zacchi
April 1998,
Biotechnology and bioengineering,
M Ehlde, and
G Zacchi
August 1967,
Experientia,
M Ehlde, and
G Zacchi
February 2010,
Mathematical biosciences,
M Ehlde, and
G Zacchi
December 1993,
The Biochemical journal,
M Ehlde, and
G Zacchi
February 2004,
Proceedings of the National Academy of Sciences of the United States of America,
M Ehlde, and
G Zacchi
July 1973,
Analytical biochemistry,
