-transparent.png){kind=logo}
The distribution of [3H]methyl radioactivity in cytoplasmic histone mRNA, isolated during the DNA synthetic (S) phase of the HeLa S3 cell cycle, has been investigated. Evidence is presented that approximately 30% of the radioactivity is in m7GpppXmpYp oligonucleotides, where Xm represents 2'-O-methylated adenosine and guanosine with a molar ratio of 4:1, respectively. The remainder of the radioactivity is present as m7GpppCmpYmpZp oligonucleotides, where Xm is again 2'-O-methylated adenosine and guanosine (4:1) and where ym represents 2'-O-methylated adenosine, guanosine, cytidine, and uridine with ratios of 2:1:1:1, respectively. While 48.6% of the [3H]methyl radioactivity was present as N6-methyladenosine in poly(adenylic acid)-terminated mRNA from S-phase cells, no evidence for N6-methyladenosine was found in histone mRNA. It thus appears that histone mRNA which lacks 3'-terminal poly(adenylic acid) sequences and functions on cytoplasmic polyribosomes during a limited protion of the cell cycle is capped but lacks internal-modified nucleosides.
J L Stein, and
G S Stein, and
P M McGuire
June 1972,
Journal of molecular biology,
J L Stein, and
G S Stein, and
P M McGuire
January 1970,
FEBS letters,
J L Stein, and
G S Stein, and
P M McGuire
February 1974,
European journal of biochemistry,
J L Stein, and
G S Stein, and
P M McGuire
September 1977,
Cell,
J L Stein, and
G S Stein, and
P M McGuire
June 1973,
Molecular biology reports,
J L Stein, and
G S Stein, and
P M McGuire
October 1972,
Biochemical and biophysical research communications,
J L Stein, and
G S Stein, and
P M McGuire
October 1977,
Proceedings of the National Academy of Sciences of the United States of America,
J L Stein, and
G S Stein, and
P M McGuire
November 1976,
Biochemical and biophysical research communications,
J L Stein, and
G S Stein, and
P M McGuire
January 1975,
Cell,
J L Stein, and
G S Stein, and
P M McGuire
April 1975,
Cell,
