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We have recently demonstrated that 17 beta-estradiol (E2) inhibits peritoneal adhesion formation. Because macrophages play a central role in inflammation and wound healing, we chose to investigate whether the E2 could inhibit the expression of JE, the murine monocyte chemoattractant protein-1 (MCP-1). To accomplish this, murine fibroblasts were cultured with physiological concentrations of E2 (3-300 pg/ml) with or without inducers of JE/MCP-1 mRNA expression. Untreated cells failed to express the message, but, following stimulation, a marked increase in JE/MCP-1 mRNA expression was observed. At 10-30 pg/ml, E2 had no effect on JE/MCP-1 mRNA expression in stimulated fibroblasts. In contrast, lower and higher doses of E2 inhibited the expression of JE/MCP-1 mRNA in stimulated fibroblasts. Treatment with tamoxifen reversed the E2-inhibition of expression of the message. These data demonstrate that JE/MCP-1 mRNA expression is controlled, in part, by estrogen and suggest that macrophage recruitment may be affected by circulating levels of E2.
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
December 1994,
Journal of immunology (Baltimore, Md. : 1950),
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
February 1995,
Journal of immunology (Baltimore, Md. : 1950),
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
April 1995,
The American journal of pathology,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
April 1993,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
June 1991,
Cellular immunology,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
January 1995,
Journal of the neurological sciences,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
June 2001,
Experimental and molecular pathology,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
October 1994,
Cancer immunology, immunotherapy : CII,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
October 2000,
Veterinary immunology and immunopathology,
E J Kovacs, and
D E Faunce, and
D S Ramer-Quinn, and
F J Mott, and
P W Dy, and
M R Frazier-Jessen
October 2002,
Pancreas,
