A simple micromethod for the direct determination of the total carbon dioxide content in biological fluids. 1977

G J Van Stekelenburg, and M H De Keyzer, and R A Donckerwolcke, and A H Maas, and R A Van De Camp

A simple method is presented for the determination of the total carbon dioxide content applicable to all biological fluids. This method makes use of a PCO2-electrode, placed in a perspex reaction/measuring chamber, connected with a IL blood gas analyzer, type 413 equiped with an electronic data-ready indicator system. The advantages of the proposed method are: 1. Depending upon the accuracy required for sera, the sample volume can be reduced to 20 or 10 micron1.2. A high degree of accuracy is obtained depending upon the sample volume used; for sera we calculated variation coefficients of 2%, 1% and 0.8% using sample volumes of 10, 20 and 30 micron 1, respectively. 3. The proposed method is simple and requires only two minutes for one determination. 4. In a comparative study an almost perfect relationship was found between the proposed method and the indirect method by calculation with a constant pK1g value.

UI MeSH Term Description Entries
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D001826 Body Fluids Liquid components of living organisms. Body Fluid,Fluid, Body,Fluids, Body
D002245 Carbon Dioxide A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals. Carbonic Anhydride,Anhydride, Carbonic,Dioxide, Carbon
D002623 Chemistry Techniques, Analytical Methodologies used for the isolation, identification, detection, and quantitation of chemical substances. Analytical Chemistry Techniques,Analytical Chemistry Methods,Analytical Chemistry Method,Analytical Chemistry Technique,Chemistry Method, Analytical,Chemistry Methods, Analytical,Chemistry Technique, Analytical,Method, Analytical Chemistry,Methods, Analytical Chemistry,Technique, Analytical Chemistry,Techniques, Analytical Chemistry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

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