BIOMAT Database Logo BIOMAT Database Logo

Successful induction of severe destructive arthritis by the transfer of in vitro-activated synovial fluid T cells from patients with rheumatoid arthritis (RA) in severe combined immunodeficient (SCID) mice. 1996

A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Department of Immunology, Kumamoto University School of Medicine, Japan.

In order to investigate the role of pathogenic T cells in RA, the establishment of an RA model using patients' T cells is thought to be essential. In this study, multiple and severe destructive arthritis was established by transferring in vitro-stimulated synovial fluid T (SFT) cells from patients with RA through simultaneous injection into knee joint and peritoneal cavity of SCID mice without causing xenogeneic graft-versus-host disease (GVHD). Neither the transfer of unstimulated SFT cells nor sole i.p. injection was sufficient to induce severe arthritis. Interestingly, in contrast with SFT cells, in vitro-activated peripheral blood lymphocytes from RA patients failed to trigger such arthritis, suggesting that pathogenic T cells might be concentrated in synovial fluid of RA patients. This, the first severe arthritis model mimicking RA induced by RA patients' T cells, is expected to provide important information about RA pathogenesis and a possible therapeutic approach.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007963 Leukocytes, Mononuclear Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules. Mononuclear Leukocyte,Mononuclear Leukocytes,PBMC Peripheral Blood Mononuclear Cells,Peripheral Blood Human Mononuclear Cells,Peripheral Blood Mononuclear Cell,Peripheral Blood Mononuclear Cells,Leukocyte, Mononuclear
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005260 Female Females
D006086 Graft vs Host Disease The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION. Graft-Versus-Host Disease,Homologous Wasting Disease,Runt Disease,Graft-vs-Host Disease,Disease, Graft-Versus-Host,Disease, Graft-vs-Host,Disease, Homologous Wasting,Disease, Runt,Diseases, Graft-Versus-Host,Diseases, Graft-vs-Host,Graft Versus Host Disease,Graft-Versus-Host Diseases,Graft-vs-Host Diseases
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly

Related Publications

A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Autoantibody production by severe combined immunodeficient mice reconstituted with synovial cells from rheumatoid arthritis patients.
August 1990, European journal of immunology,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Successful transfer of collagen-induced arthritis to severe combined immunodeficient (SCID) mice.
June 1992, Clinical and experimental immunology,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Orthotopic implantation of inflamed synovial tissue from RA patients induces a characteristic arthritis in immunodeficient (SCID) mice.
February 1996, Journal of autoimmunity,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
The SCID-ra mouse: rat T-cell differentiation in the severe combined immunodeficient mouse.
June 1993, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Adoptive transfer of susceptibility to antigen-induced arthritis into severe combined immunodeficient (SCID) mice: role of CD4+ and CD8+ T cells.
July 1996, Immunological investigations,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
CD4+ T cells from collagen-induced arthritic mice are essential to transfer arthritis into severe combined immunodeficient mice.
August 1994, Clinical and experimental immunology,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Transfer of human systemic lupus erythematosus in severe combined immunodeficient (SCID) mice.
September 1990, The Journal of experimental medicine,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Markers of activated T cells on synovial fluid lymphocytes in rheumatoid arthritis.
January 1979, Bollettino dell'Istituto sieroterapico milanese,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment.
January 2003, Methods in molecular medicine,
A Sakata, and K Sakata, and H Ping, and T Ohmura, and M Tsukano, and K Kakimoto
Adriamycin nephropathy in severe combined immunodeficient (SCID) mice.
November 2006, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
Copied contents to your clipboard!