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The objective of this study was to develop a highly specific enzyme-linked immunosorbent assay (ELISA) for the serological detection of anti-Ornithodoros tick antibodies in animals. Affinity-purified rabbit anti-Ornithodoros IgG antibodies were employed in indirect competitive inhibition ELISA assays designed to measure the anti-Ornithodoros antibody titers in other animal species using the domestic goat (Capra hircus) as a large animal model. Repeated infestation of goats with Ornithodoros coriaceus was found to elicit the formation of antibodies capable of inhibiting the binding of the Ornithodoros-specific rabbit IgG. Western blot analysis of goat and rabbit anti-tick antisera demonstrated both animal species to respond immunologically to a set of 9 major protein bands in O. coriaceus salivary gland extracts. The results of these experiments demonstrate that a history of animal exposure to O. coriaceus may be detected serologically by competitive inhibition ELISA.
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
April 1990,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
March 2017,
Biologicals : journal of the International Association of Biological Standardization,
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
November 2006,
Xenotransplantation,
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
January 2023,
Methods in molecular biology (Clifton, N.J.),
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
June 1990,
The Southeast Asian journal of tropical medicine and public health,
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
January 2022,
The journal of applied laboratory medicine,
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
January 1992,
Revue roumaine de virologie (Bucharest, Romania : 1990),
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
June 2011,
Journal of virological methods,
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
January 2022,
Methods in molecular biology (Clifton, N.J.),
E J Wozniak, and
J F Butler, and
R G Endris, and
S G Zam
December 1988,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
