-transparent.png){kind=logo}
Changes in distribution of silver grains were evaluated in autoradiograms of nuclei of synchronized HeLa cells that were continuously labelled with 3H-TdR. Synchronization was achieved by repeated blocking with cold thymidine. Three patterns of grain distribution were distinguished: uniform, non-uniform, and a special case of non-uniform distribution, the S.C. clustering. The kinetics of changes in the proportion of individual types of grain distribution indicate that uniform and non-uniform distribution patterns result from 3H-TdR incorporation into DNA replicating during the entire S-phase, whereas DNA responsible for clustered grain distribution takes up 3H-TdR only during the second half of the S-phase. The rate of the increase in grain counts per nucleus of labelled cell and the kinetics of the exhaustion of radioactive precursor from the culture medium suggest that replication of DNA in DNA in the S-phase is not merely a linear function of time.
L Benes, and
E Rotreklová
June 1977,
Histochemistry,
L Benes, and
E Rotreklová
December 1988,
Tsitologiia,
L Benes, and
E Rotreklová
January 1977,
Developments in biological standardization,
L Benes, and
E Rotreklová
September 1969,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
L Benes, and
E Rotreklová
April 1971,
Journal of molecular biology,
L Benes, and
E Rotreklová
April 1974,
Doklady Akademii nauk SSSR,
L Benes, and
E Rotreklová
April 1967,
Experimental cell research,
L Benes, and
E Rotreklová
August 1965,
Biochemical and biophysical research communications,
L Benes, and
E Rotreklová
January 1968,
Acta histochemica,
L Benes, and
E Rotreklová
January 1966,
Die Naturwissenschaften,
