-transparent.png){kind=logo}
Five transplantable tumors, which originated from human maligant neoplasms and had been maintained in nude mice, were frozen at--70 degrees preserved for 1226 days. Tumor tissue was minced in a mixture of tissue culture medium, 10% dimethyl sulfoxide, and 10% calf serum before freezing. The transplantability of frozen tumors was examined after thawing. Morphological and functional characteristic of each tumor were compared before and after the frozen storage. The results suggest that low-temperature preservation technique can be applied satisfactorily for the maintenance of human malignant tumors which have been maintained by using nude mice.
Related Publications
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
April 1983,
The Japanese journal of experimental medicine,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
January 1980,
Experimental cell biology,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
January 1988,
In vivo (Athens, Greece),
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
January 1985,
Oncology,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
January 1982,
Jikken dobutsu. Experimental animals,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
September 1984,
No to shinkei = Brain and nerve,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
December 1982,
Gan,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
October 1988,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
December 1983,
The Tokai journal of experimental and clinical medicine,
Y Ueyama, and
M Yokoyama, and
Y Kondo, and
N Ohsawa, and
N Tamoki
July 1986,
Nihon Gan Chiryo Gakkai shi,
