-transparent.png){kind=logo}
The alternative nitrogenase from a nifH mutant of the photosynthetic bacterium Rhodospirillum rubrum has been purified and characterized. The dinitrogenase protein (ANF1) contains three subunits in an apparent alpha2beta2gamma2 structure and contains Fe but no Mo or V. A factor capable of activating apo-dinitrogenase (lacking the FeMo cofactor) from Azotobacter vinelandii was extracted from the alternative dinitrogenase protein with N-methylformamide. The electron paramagnetic resonance (EPR) signal of the dinitrogenase protein is not characteristic of the EPR signals of molybdenum- or vanadium-containing dinitrogenases. The alternative dinitrogenase reductase (ANF2) was purified as an alpha2 dimer containing an Fe4S4 cluster and exhibited an EPR spectrum characteristic of dinitrogenase reductases. The enzyme complex reduces protons to H2 very well but reduces N2 to ammonium poorly. Acetylene is reduced to a mixture of ethylene and ethane.
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
February 1979,
Journal of bacteriology,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
November 1978,
Biochimica et biophysica acta,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
February 1989,
Biochimica et biophysica acta,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
June 1981,
Journal of biochemistry,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
December 1984,
The Journal of biological chemistry,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
June 1949,
Science (New York, N.Y.),
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
January 1980,
Journal of biochemistry,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
October 1979,
Journal of biochemistry,
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
April 2006,
Journal of microbiology (Seoul, Korea),
R Davis, and
L Lehman, and
R Petrovich, and
V K Shah, and
G P Roberts, and
P W Ludden
September 2007,
Journal of microbiology and biotechnology,
