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In order to investigate T cell recognition of allergens in hen egg allergy, we have established 30 ovalbumin (OVA)-specific T cell lines (TCLs) from peripheral blood mononuclear cells of 6 patients with atopic dermatitis, who are positive for IgE antibodies to OVA and clinically allergic to hen egg, and characterized them for their cytokine production pattern. All TCLs we could study were mainly composed of CD4+ T cells. Most TCLs produced significant amounts of interleukin 4 (IL-4) and IL-5 but no or very little interferon gamma on antigen stimulation, suggesting that these TCLs belong to TH2-type T cells. Restriction elements and epitope specificities were further studied on some TCLs. Antibody blocking of the proliferative responses of the TCLs to OVA indicated that HLA-DR are acting as the dominant restriction elements for these TCLs with minor contribution of HLA-DQ. By use of 187 overlapping synthetic peptides covering the whole sequence of OVA, at least 3 different T cell epitopes were identified.
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
January 2005,
Journal of investigational allergology & clinical immunology,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
September 1996,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
February 1996,
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
October 2001,
Oral microbiology and immunology,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
October 1983,
Proceedings of the National Academy of Sciences of the United States of America,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
June 1983,
The Japanese journal of experimental medicine,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
October 1989,
Arerugi = [Allergy],
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
September 1984,
The British journal of dermatology,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
May 2002,
Journal of dental research,
T Katsuki, and
N Shimojo, and
K Honma, and
H Tsunoo, and
Y Kohno, and
H Niimi
October 1994,
Clinical immunology and immunopathology,
