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The effects of adenosine (ADO) analogs on cells of the human promyelocytic HL-60 line were examined. ADO A(3) receptor agonists, N(6)-(3-iodobenzyl)adenosine-5'-N-methylcarboxamide (IB-MECA, 30-60 microM) and 2-chloro-N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (CI-IB-MECA, 10-30 microM) induced apoptotic cell death. In contrast, neither an A(1)/A(2) antagonist (XAC) nor other selective ADO receptor agonists (CPA, NECA and CGS21680) induced apoptosis at concentrations of <30 microM. Both IB-MECA and CI-IB-MECA significantly induced Ca(2+) release from intracellular Ca(2+) pools followed by Ca(2+) influx, suggesting the presence of phospholipase C-coupled ADO A(3) receptors on HL-60 cells. This was further supported by the presence of mRNA of ADO A3 receptor in the cells. These results suggest that activation of ADO A(3) receptors is responsible for the ADO-induced apoptosis in HL-60 cells and could be of potential therapeutic value in the treatment of leukemia.
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
September 2009,
Toxicological research,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
January 2002,
Anticancer research,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
February 2003,
Archives of pharmacal research,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
January 2004,
In vivo (Athens, Greece),
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
March 1995,
Biochemical and biophysical research communications,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
July 1994,
Experimental cell research,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
April 2005,
Planta medica,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
July 2004,
Biological & pharmaceutical bulletin,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
March 2004,
Cancer letters,
Y Kohno, and
Y Sei, and
M Koshiba, and
H O Kim, and
K A Jacobson
March 2002,
Environmental toxicology and pharmacology,
