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Alpha 2A-adrenergic regulation of cyclic AMP accumulation and lipolysis in human omental and subcutaneous adipocytes. 1996

H L Vikman, and J M Savola, and A Raasmaja, and J J Ohisalo
Department of Medical Chemistry, University of Helsinki, Finland.

OBJECTIVE To characterize differences in alpha 2-adrenergic regulation between subcutaneous and omental adipocytes which could offer a possibility of pharmacological intervention in the metabolic syndrome. METHODS Both subcutaneous and omental adipocytes were isolated from 32 patients. Adipocytes were incubated in the presence of adrenoceptor agonists, and cyclic AMP and glycerol levels were measured. alpha 2-Adrenoceptors of isolated plasma membranes were characterized. RESULTS Adrenaline increased cyclic AMP levels about two-fold in omental adipocytes but had almost no effect in subcutaneous fat cells. The inhibition of cyclic AMP accumulation and glycerol release by UK-14304 and dexmedetomidine was less pronounced in omental adipocytes. The maximal effect of isoprenaline on cyclic AMP levels and glycerol release was similar at the two sites. The subcutaneous and omental alpha-adrenoceptors had similar affinities to 3H-RX821002 and showed characteristics of the alpha 2A subtype. The receptor densities were 220 +/- 21 and 460 +/- 84 fmol/mg of protein (means +/- s.e.m.) in omental and subcutaneous membranes, respectively. CONCLUSIONS Inhibition of cyclic AMP accumulation and lipolysis by alpha 2A-adrenoceptors is less pronounced in omental than subcutaneous adipocytes which could be due to differences in receptor number. These differences in alpha 2A-adrenergic regulation could be of value in the treatment of the metabolic syndrome.

UI MeSH Term Description Entries
D007093 Imidazoles Compounds containing 1,3-diazole, a five membered aromatic ring containing two nitrogen atoms separated by one of the carbons. Chemically reduced ones include IMIDAZOLINES and IMIDAZOLIDINES. Distinguish from 1,2-diazole (PYRAZOLES).
D007545 Isoproterenol Isopropyl analog of EPINEPHRINE; beta-sympathomimetic that acts on the heart, bronchi, skeletal muscle, alimentary tract, etc. It is used mainly as bronchodilator and heart stimulant. Isoprenaline,Isopropylarterenol,4-(1-Hydroxy-2-((1-methylethyl)amino)ethyl)-1,2-benzenediol,Euspiran,Isadrin,Isadrine,Isopropyl Noradrenaline,Isopropylnoradrenaline,Isopropylnorepinephrine,Isoproterenol Hydrochloride,Isoproterenol Sulfate,Isuprel,Izadrin,Norisodrine,Novodrin,Hydrochloride, Isoproterenol,Noradrenaline, Isopropyl,Sulfate, Isoproterenol
D008066 Lipolysis The metabolic process of breaking down LIPIDS to release FREE FATTY ACIDS, the major oxidative fuel for the body. Lipolysis may involve dietary lipids in the DIGESTIVE TRACT, circulating lipids in the BLOOD, and stored lipids in the ADIPOSE TISSUE or the LIVER. A number of enzymes are involved in such lipid hydrolysis, such as LIPASE and LIPOPROTEIN LIPASE from various tissues. Lipolyses
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009852 Omentum A double-layered fold of peritoneum that attaches the STOMACH to other organs in the ABDOMINAL CAVITY. Omentums
D011810 Quinoxalines Quinoxaline
D011942 Receptors, Adrenergic, alpha One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation. Adrenergic alpha-Receptor,Adrenergic alpha-Receptors,Receptors, alpha-Adrenergic,alpha-Adrenergic Receptor,alpha-Adrenergic Receptors,Receptor, Adrenergic, alpha,Adrenergic alpha Receptor,Adrenergic alpha Receptors,Receptor, alpha-Adrenergic,Receptors, alpha Adrenergic,alpha Adrenergic Receptor,alpha Adrenergic Receptors,alpha-Receptor, Adrenergic,alpha-Receptors, Adrenergic
D004837 Epinephrine The active sympathomimetic hormone from the ADRENAL MEDULLA. It stimulates both the alpha- and beta- adrenergic systems, causes systemic VASOCONSTRICTION and gastrointestinal relaxation, stimulates the HEART, and dilates BRONCHI and cerebral vessels. It is used in ASTHMA and CARDIAC FAILURE and to delay absorption of local ANESTHETICS. Adrenaline,4-(1-Hydroxy-2-(methylamino)ethyl)-1,2-benzenediol,Adrenaline Acid Tartrate,Adrenaline Bitartrate,Adrenaline Hydrochloride,Epifrin,Epinephrine Acetate,Epinephrine Bitartrate,Epinephrine Hydrochloride,Epinephrine Hydrogen Tartrate,Epitrate,Lyophrin,Medihaler-Epi,Acetate, Epinephrine
D005260 Female Females

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