Unilateral enucleation induces an increase of 160 kd neurofilament in lateral geniculate nuclei synapses. 1996

F Capani, and C F Loidl, and J Pecci Saavedra
Instituto de Biología Celular y Neurociencias Prof. Eduardo De Robertis, Facultad de Medicina, Universidad de Buenos Aires, República Argentina.

Ultrastructural synaptic changes of retinal origin in the pars dorsalis lateral geniculate nuclei (dLGN) after enucleation have been studied in this laboratory, showing a filamentous hypertrophy with maximal expression at 4-6 days post-lesion in monkeys (Pecci Saavedra et al., 1970, 1971). The aim of this work was to elucidate the nature of the newly formed filament in dLGN in post-enucleated rats. Male Wistar rats were fixed with 4% paraformaldehyde plus 0.25% glutaraldehyde in 0.1M phosphate buffer, through the abdominal aorta after 3, 5, and 7 days postenucleation. Sections obtained were incubated with antibodies to the phosphorylated portion of the 160 Kd neurofilaments (1:3000) and anti-GFAP (1:25000). There was an increase in 160 Kd neurofilament staining in axons and degenerating nerve endings in dLGN, as well as a typical astroglial immunostained reaction. Our results show that the newly formed neurofilaments after deafferentation are of the 160 Kd type, commonly present in normal axons.

UI MeSH Term Description Entries
D008297 Male Males
D009410 Nerve Degeneration Loss of functional activity and trophic degeneration of nerve axons and their terminal arborizations following the destruction of their cells of origin or interruption of their continuity with these cells. The pathology is characteristic of neurodegenerative diseases. Often the process of nerve degeneration is studied in research on neuroanatomical localization and correlation of the neurophysiology of neural pathways. Neuron Degeneration,Degeneration, Nerve,Degeneration, Neuron,Degenerations, Nerve,Degenerations, Neuron,Nerve Degenerations,Neuron Degenerations
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D005829 Geniculate Bodies Part of the DIENCEPHALON inferior to the caudal end of the dorsal THALAMUS. Includes the lateral geniculate body which relays visual impulses from the OPTIC TRACT to the calcarine cortex, and the medial geniculate body which relays auditory impulses from the lateral lemniscus to the AUDITORY CORTEX. Lateral Geniculate Body,Medial Geniculate Body,Metathalamus,Corpus Geniculatum Mediale,Geniculate Nucleus,Lateral Geniculate Nucleus,Medial Geniculate Complex,Medial Geniculate Nucleus,Nucleus Geniculatus Lateralis Dorsalis,Nucleus Geniculatus Lateralis Pars Dorsalis,Bodies, Geniculate,Complex, Medial Geniculate,Complices, Medial Geniculate,Corpus Geniculatum Mediales,Geniculate Bodies, Lateral,Geniculate Bodies, Medial,Geniculate Body,Geniculate Body, Lateral,Geniculate Body, Medial,Geniculate Complex, Medial,Geniculate Complices, Medial,Geniculate Nucleus, Lateral,Geniculate Nucleus, Medial,Geniculatum Mediale, Corpus,Geniculatum Mediales, Corpus,Lateral Geniculate Bodies,Medial Geniculate Bodies,Medial Geniculate Complices,Mediale, Corpus Geniculatum,Mediales, Corpus Geniculatum,Nucleus, Geniculate,Nucleus, Lateral Geniculate,Nucleus, Medial Geniculate
D005904 Glial Fibrillary Acidic Protein An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000. Glial Intermediate Filament Protein,Astroprotein,GFA-Protein,Glial Fibrillary Acid Protein,GFA Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001253 Astrocytes A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury. Astroglia,Astroglia Cells,Astroglial Cells,Astrocyte,Astroglia Cell,Astroglial Cell,Astroglias,Cell, Astroglia,Cell, Astroglial
D013569 Synapses Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions. Synapse
D015353 Eye Enucleation The surgical removal of the eyeball leaving the eye muscles and remaining orbital contents intact. Enucleation Surgery,Enucleation Technique,Enucleation Surgeries,Enucleation Techniques,Enucleation, Eye,Eye Enucleations,Surgery, Enucleation
D016900 Neurofilament Proteins Type III intermediate filament proteins that assemble into neurofilaments, the major cytoskeletal element in nerve axons and dendrites. They consist of three distinct polypeptides, the neurofilament triplet. Types I, II, and IV intermediate filament proteins form other cytoskeletal elements such as keratins and lamins. It appears that the metabolism of neurofilaments is disturbed in Alzheimer's disease, as indicated by the presence of neurofilament epitopes in the neurofibrillary tangles, as well as by the severe reduction of the expression of the gene for the light neurofilament subunit of the neurofilament triplet in brains of Alzheimer's patients. (Can J Neurol Sci 1990 Aug;17(3):302) Neurofilament Protein,Heavy Neurofilament Protein,Neurofilament Triplet Proteins,Neurofilament Protein, Heavy,Protein, Heavy Neurofilament,Protein, Neurofilament,Proteins, Neurofilament,Proteins, Neurofilament Triplet,Triplet Proteins, Neurofilament

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