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A system of cell-mediated mutagenesis is described for the study of compounds which require metabolic activation to exert their cytotoxic and mutagenic effects. This system combines BHK21 cells for metabolism of the compounds and V79 cells as targets for mutagenesis. Using the two polycyclic hydrocarbon carcinogens benzo(a)pyrene and 7-methylbenz(a)anthracene we have shown that the hydrocarbon-DNA reaction which accompanies mutagenesis in the target cell is indistinguishable from that reported to occur in vivo and in primary cell cultures. Our results also support the view that a diol epoxide metabolite is responsible for the biological activity of benzo(a)pyrene. The application of cell-mediated mutagenesis to the routine testing of suspect environmental chemicals for biological activity is discussed.
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
August 1975,
Mutation research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
February 1977,
Mutation research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
October 1983,
Mutation research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
May 1970,
Cancer research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
September 1981,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
November 1982,
Cancer research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
April 1973,
Mutation research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
April 1973,
Cancer research,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
April 1970,
Nature,
R F Newbold, and
C B Wigley, and
M H Thompson, and
P Brookes
April 1978,
Proceedings of the National Academy of Sciences of the United States of America,
