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The gene encoding dihydrolipoamide dehydrogenase from the halophilic archaeon, Haloferax volcanii, has been subcloned and overexpressed in the parent organism by using the halophilic archaeal rRNA promoter. The recombinant protein has been purified to homogeneity and characterized with respect to its kinetic, molecular, and salt-dependent properties. A dihydrolipoamide dehydrogenase-minus mutant of H. volcanii has been created by homologous recombination with the subcloned gene after insertion of the mevinolin resistance determinant into the protein-coding region. To explore the physiological function of the dihydrolipoamide dehydrogenase, the growth properties of the mutant halophile have been examined.
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
September 1997,
European journal of biochemistry,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
January 1992,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
January 2000,
Archives of microbiology,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
October 2020,
Proceedings of the National Academy of Sciences of the United States of America,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
February 2009,
Biochemical Society transactions,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
April 2002,
FEMS microbiology letters,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
October 2004,
Extremophiles : life under extreme conditions,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
August 1993,
Biochemical and biophysical research communications,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
December 2002,
FEBS letters,
K A Jolley, and
E Rapaport, and
D W Hough, and
M J Danson, and
W G Woods, and
M L Dyall-Smith
October 2001,
The Journal of membrane biology,
