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Chemically synthesized lactose operator DNA was tested for binding with lactose repressor protein. These operator DNAs were found to (1) bind specifically to lactose SQ repressor as measured by release of binding with the inducing ligand isopropyl-beta-D-thiogalactoside, (2) have dissociation half-lives of 37 seconds (21 base-paired duplex) and 46 seconds (26 base-paired duplex) and (3) have dissociation half-lives with x86 repressor of 9 minutes (21 base-paired duplex) and 18 minutes (26 base paired duplex). Modified operators containing 5-bromodeoxyuridine and deoxyuridine at specific sites were also prepared. These analogs bound both repressors about as tightly as the wild type sequence.
D G Yansura, and
D V Goeddel, and
D L Cribbs, and
M H Caruthers
March 1972,
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D G Yansura, and
D V Goeddel, and
D L Cribbs, and
M H Caruthers
September 1982,
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D G Yansura, and
D V Goeddel, and
D L Cribbs, and
M H Caruthers
December 1992,
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D G Yansura, and
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M H Caruthers
December 1970,
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D G Yansura, and
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June 1979,
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D G Yansura, and
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M H Caruthers
January 1975,
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D G Yansura, and
D V Goeddel, and
D L Cribbs, and
M H Caruthers
January 1977,
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D G Yansura, and
D V Goeddel, and
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M H Caruthers
May 1974,
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D G Yansura, and
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D G Yansura, and
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