BIOMAT Database Logo BIOMAT Database Logo

Soluble form of Lamp II in purified rat liver lysosomes. 1996

M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Laboratoire de Chimie Physiologique, Facultes Universitairés Notre Dame de la Paix, Namur, Belgium. michel.iadot@fundp.ac.be

Lamp II (for lysosomal associated membrane protein II) is an integral type I glycoprotein. It consists of a very large and heavily glycosylated luminal domain, a single transmembrane segment, and a short cytoplasmic tail. We show that in highly purified lysosomes from rat liver, Lamp II, immunodetected with a monoclonal antibody on Western blots, it surprisingly distributed between a membrane bound form and a "soluble" form. The partition of the protein between the membrane and the content of lysosomes is strongly pH dependent. The soluble Lamp II population is sensitive to pH dependent aggregation as it is for many lysosomal content enzymes.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008247 Lysosomes A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured or undergoes MEMBRANE FUSION. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed). Autolysosome,Autolysosomes,Lysosome
D008297 Male Males
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D002458 Cell Fractionation Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS. Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings

Related Publications

M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Cystine transport in purified rat liver lysosomes.
June 1986, The Biochemical journal,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Nucleoside diphosphatase in purified preparations of rat liver lysosomes.
December 1969, FEBS letters,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Large-scale preparation of highly purified lysosomes from normal rat liver.
February 1980, Analytical biochemistry,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Isolation of deoxyribonuclease II of rat liver lysosomes.
March 1972, The Journal of biological chemistry,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Soluble and membrane-bound enzyme-active antigens of rat-liver lysosomes.
February 1975, European journal of biochemistry,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Distribution of dipeptidase activity between lysosomes and soluble fraction of rat liver.
May 1975, Canadian journal of biochemistry,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
Purification and characterization of a soluble form of lysosome-associated membrane glycoprotein-2 (lamp-2) from rat liver lysosomal contents.
September 1998, Biochemistry and molecular biology international,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
[Isolation of a purified preparation of rat liver lysosomes by metrizamide gradient centrifugation (proceedings)].
December 1977, Archives internationales de physiologie et de biochimie,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
The synthesis of rat liver lysosomes. III. Chemical composition of microsomal and lysosomal beta-glucuronidases purified from rat liver.
February 1978, Journal of biochemistry,
M Jadot, and R Wattiaux, and F Mainferme, and F Dubois, and A Claessens, and S Wattiaux-De Coninck
A simple procedure for the isolation of highly purified lysosomes from normal rat liver.
April 1984, Journal of biochemistry,
Copied contents to your clipboard!