BIOMAT Database Logo BIOMAT Database Logo

Binding reactivity of monoclonal anti-carcinoembryonic antigen (CEA) antibodies with cell membrane-bound CEA and with free CEA in solution. 1996

M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
First Department of Biochemistry, School of Medicine, Fukuoka University, Fukuoka, Japan.

Binding reactivities of 62 anti-CEA MAbs from 10 different research groups with cell membrane-bound CEA and with free CEA in solution were compared by inhibition of MAb binding to CEA-expressing tumor cells by free CEA. Bindings of 30 MAbs to the cell membrane-bound CEA (280 ng CEA/2 x 10(5) cells) were inhibited by approximately equal amounts of free CEA, indicating that binding affinities of about half the MAbs for cell membrane-bound CEA are similar to those for free CEA, respectively. Bindings of 15 MAbs to the cell membrane-bound CEA were easily inhibited by free CEA of less than half the amount of the cell membrane-bound CEA, while inhibition of bindings of the remaining 17 MAbs required twice more free CEA than the amount of cell membrane-bound CEA, showing that about one-fourth of the MAbs have higher affinities for free CEA and the remaining about one-fourth of the MAbs possess higher affinities for cell membrane-bound CEA. These results help form the basis for selecting the anti-CEA MAbs for use in clinical applications, such as serum CEA assay, tumor imaging and immunotherapy.

UI MeSH Term Description Entries
D008113 Liver Neoplasms Tumors or cancer of the LIVER. Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D002272 Carcinoembryonic Antigen A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment. Antigens, CD66e,CD66e Antigen,Antigen, CD66e,Antigen, Carcinoembryonic,CD66e Antigens
D003110 Colonic Neoplasms Tumors or cancer of the COLON. Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000230 Adenocarcinoma A malignant epithelial tumor with a glandular organization. Adenocarcinoma, Basal Cell,Adenocarcinoma, Granular Cell,Adenocarcinoma, Oxyphilic,Adenocarcinoma, Tubular,Adenoma, Malignant,Carcinoma, Cribriform,Carcinoma, Granular Cell,Carcinoma, Tubular,Adenocarcinomas,Adenocarcinomas, Basal Cell,Adenocarcinomas, Granular Cell,Adenocarcinomas, Oxyphilic,Adenocarcinomas, Tubular,Adenomas, Malignant,Basal Cell Adenocarcinoma,Basal Cell Adenocarcinomas,Carcinomas, Cribriform,Carcinomas, Granular Cell,Carcinomas, Tubular,Cribriform Carcinoma,Cribriform Carcinomas,Granular Cell Adenocarcinoma,Granular Cell Adenocarcinomas,Granular Cell Carcinoma,Granular Cell Carcinomas,Malignant Adenoma,Malignant Adenomas,Oxyphilic Adenocarcinoma,Oxyphilic Adenocarcinomas,Tubular Adenocarcinoma,Tubular Adenocarcinomas,Tubular Carcinoma,Tubular Carcinomas
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000915 Antibody Affinity A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes. Affinity, Antibody,Antibody Avidity,Avidity, Antibody,Affinities, Antibody,Antibody Affinities,Antibody Avidities,Avidities, Antibody
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000937 Antigen-Antibody Reactions The processes triggered by interactions of ANTIBODIES with their ANTIGENS. Antigen Antibody Reactions,Antigen-Antibody Reaction,Reaction, Antigen-Antibody,Reactions, Antigen-Antibody

Related Publications

M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Impact of Different Selectivity between Soluble and Membrane-bound Forms of Carcinoembryonic Antigen (CEA) on the Target-mediated Disposition of Anti-CEA Monoclonal Antibodies.
November 2019, Drug metabolism and disposition: the biological fate of chemicals,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Characterization of a variant of carcinoembryonic antigen (CEA) using monoclonal and polyclonal anti-CEA antibodies.
December 1982, Biochemical and biophysical research communications,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Detection of carcinoembryonic antigen in colonic effluent by specific anti-CEA monoclonal antibodies.
October 1992, Cancer letters,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Epitopes of carcinoembryonic antigen (CEA) defined by monoclonal antibodies.
February 1988, British journal of cancer,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
[Clinical application of carcinoembryonic antigen (CEA) monoclonal antibodies (McAbs)].
January 1987, Zhonghua zhong liu za zhi [Chinese journal of oncology],
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA).
January 1991, Biomedica biochimica acta,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Specificity of anti-carcinoembryonic antigen monoclonal antibodies and their effects on CEA-mediated adhesion.
August 1993, Cancer research,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Kinetic and affinity constants of epitope specific anti-carcinoembryonic antigen (CEA) monoclonal antibodies for CEA and engineered CEA domain constructs.
June 1998, Immunotechnology : an international journal of immunological engineering,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Immunological characteristics of monoclonal antibodies against human carcinoembryonic antigen (CEA).
October 1985, Proceedings of the National Science Council, Republic of China. Part B, Life sciences,
M Murakami, and M Kuroki, and F Arakawa, and M Haruno, and M Kuwahara, and H Ozaki, and T Senba, and Y Matsuoka
Monoclonal antibodies to carcinoembryonic antigen (CEA): characterization and use in a radioimmunoassay for CEA.
June 1982, Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology,
Copied contents to your clipboard!