Biomaterial Database

Subunit orientation in the filamentous virus Ff(fd, f1, M13). 1996

S A Overman, and M Tsuboi, and G J Thomas

Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, 64110, USA.

The Ff filamentous viruses (fd, M13, f1) are important models for membrane protein assembly and are used extensively as cloning vectors and vehicles for peptide display. The thread-like virion (approximately 6 nm x 880 nm) comprises a single-stranded DNA genome sheathed by approximately 2700 copies of a 50-residue alpha-helical subunit (pVIII). The average inclination of pVIII subunits from the virion axis has been determined experimentally by polarized Raman microspectroscopy of oriented fd fibers, using the amide I Raman tensors obtained from a peptide single crystal. It is found that the average tilt angle, theta, between the pVIII alpha-helix and the virion axis falls within the range 13< theta <20 degrees.

UI	MeSH Term	Description	Entries
D008958	Models, Molecular	Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.	Molecular Models,Model, Molecular,Molecular Model
D013059	Spectrum Analysis, Raman	Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.	Raman Spectroscopy,Analysis, Raman Spectrum,Raman Optical Activity Spectroscopy,Raman Scattering,Raman Spectrum Analysis,Scattering, Raman,Spectroscopy, Raman
D014758	Viral Core Proteins	Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.	Core Proteins, Viral,Major Core Protein,Major Core Proteins, Viral,Adenovirus Core Protein VII,Core Protein V,Core Protein lambda 2,Influenza Virus Core Proteins,Major Core Protein lambda 1,Major Core Protein lambda-1,Major Core Protein sigma 2,Major Core Protein sigma-2,OVP 19,Oncornaviral Protein P19,P30 Core Proteins,Viral Protein P19,Virus Core Proteins,Core Protein, Major,Core Proteins, P30,Core Proteins, Virus,Protein P19, Oncornaviral,Protein P19, Viral,Protein, Major Core,Proteins, P30 Core,Proteins, Viral Core,Proteins, Virus Core
D014771	Virion	The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.	Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D017104	Bacteriophage M13	Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.	Bacteriophage fd,Coliphage M13,Coliphage fd,Enterobacteria phage M13,Enterobacteria phage fd,M13 Phage,Phage M13,Phage fd,fd Phage,M13 Phages,M13, Coliphage,Phage, M13,Phage, fd,Phages, M13,Phages, fd,fd Phages
D017433	Protein Structure, Secondary	The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to ALPHA-HELICES; BETA-STRANDS (which align to form BETA-SHEETS), or other types of coils. This is the first folding level of protein conformation.	Secondary Protein Structure,Protein Structures, Secondary,Secondary Protein Structures,Structure, Secondary Protein,Structures, Secondary Protein
D017912	Inovirus	A genus of filamentous bacteriophages of the family INOVIRIDAE. Organisms of this genus infect enterobacteria, PSEUDOMONAS; VIBRIO; and XANTHOMONAS.	Bacteriophages, Filamentous,Filamentous Bacteriophages,Bacteriophage, Filamentous,Filamentous Bacteriophage,Inoviruses