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A long term culture of aortic medial cells, derived from newborn pig aorta, has been established. The ability of these cells to synthesize soluble elastin has been demonstrated by isolation and characterization of the radioactively labeled protein, using soluble elastin of copper-deficient pig aorta as the carrier. The synthesis of corsslinked elastin was shown by the isolation and identification of the lysine-derived crosslinks after incubating the cultures with [14C]-labeled lysine. The precursor relationship of soluble to insoluble elastin was demonstrated by incubating the [3H]-labeled soluble elastin from copper-deficient pig aorta with the culture and isolating labeled crosslinks from the insoluble elastin residue.
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
October 1979,
Biochemical and biophysical research communications,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
November 1975,
Biochemical and biophysical research communications,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
June 1974,
Biochemical and biophysical research communications,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
January 1982,
Methods in enzymology,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
August 1980,
Biochemical and biophysical research communications,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
December 1973,
The Journal of biological chemistry,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
October 1969,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
June 1981,
Biochemistry,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
April 1981,
Biochemistry,
P A Abraham, and
M L Hart, and
A R Winge, and
W H Carnes
March 1986,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
