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Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma. 1995
T Nomura, and
M Ohtsuki, and
S Matsui, and
C Sumi-Ichinose, and
H Nomura, and
Y Hagino, and
K Iwase, and
H Ichinose, and
K Fujita, and
T Nagatsu
Department of Pharmacology, Fujita Health University School of Medicine, Aichi, Japan.
Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single mRNA species of 4.0 kb in human pheochromocytoma tissue.
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