-transparent.png){kind=logo}
The effect of carbachol on Trypanosoma cruzi epimastigote forms was studied. Suspension of epimastigote cells prelabelled with myo[3H]-inositol were incubated at 28 degrees C in the presence of carbachol. The levels of inositol phosphate after the challenge of carbachol were determined to see if the accumulation of inositol phosphate was time-dependent. In the presence of Li+ the levels of IP3 and IP4 were significantly increased during the 3 min. with a peak at 1 min. after the carbachol challenge. This effect was suppressed by atropine. The results show that carbachol influences rapid changes in phosphoinositide metabolism and they are time-dependent involving second-messenger systems associated with the generation of IP3. In addition they would suggest that this IP3 generation is through a receptor-mediated process linked to phospholipase C by a G-protein.
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
July 1996,
Neuroscience letters,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
April 1991,
The Biochemical journal,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
August 1990,
Hearing research,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
January 1985,
Nature,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
November 1989,
Experimental parasitology,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
October 1972,
Experientia,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
January 1986,
Neuropeptides,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
February 2021,
ACS chemical biology,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
January 1968,
Revista de la Sociedad Argentina de Biologia,
M N Garrido, and
M I Bollo, and
E E Machado-Domenech
May 2004,
FEMS microbiology letters,
